Ruiz-Gonzalvo F, Rodríguez F, Escribano J M
Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, Madrid, 28130, Spain.
Virology. 1996 Apr 1;218(1):285-9. doi: 10.1006/viro.1996.0193.
A recombinant baculovirus harboring the hemagglutinin (HA) gene of African swine fever virus, with homology to the T-lymphocyte surface antigen CD2, was constructed. The efficient expression of the HA gene was determined by immunofluorescence and Western blot studies on insect cells infected with the recombinant baculovirus. The baculovirus-expressed HA showed hemadsorption and erythrocyte-agglutinating activities characteristic of the CD2 homolog protein induced by the virus in infected macrophages. Pigs immunized with the recombinant HA developed hemagglutination-inhibition and temporary infection-inhibition antibodies that recognize a 75-kDa structural protein and were protected against lethal infection.
构建了一种携带非洲猪瘟病毒血凝素（HA）基因的重组杆状病毒，该基因与T淋巴细胞表面抗原CD2具有同源性。通过对感染重组杆状病毒的昆虫细胞进行免疫荧光和蛋白质印迹研究，确定了HA基因的高效表达。杆状病毒表达的HA表现出由病毒在感染巨噬细胞中诱导的CD2同源蛋白所特有的血细胞吸附和红细胞凝集活性。用重组HA免疫的猪产生了血凝抑制和临时感染抑制抗体，这些抗体识别一种75 kDa的结构蛋白，并对致死性感染具有保护作用。
