Andersson T, Wårdell K, Anderson C
Department of Dermatology, University Hospital, Linköping, Sweden.
Acta Derm Venereol. 1995 Sep;75(5):343-7. doi: 10.2340/0001555575343347.
A novel bioanalytical in vivo sampling technique, cutaneous microdialysis, was used to follow the chronology of skin histamine release in 3 patients with cold urticaria and in 2 healthy volunteers. Laser Doppler perfusion imaging was used simultaneously to monitor the skin circulatory response. Microdialysis samples were collected at 10-min intervals and analysed by radioimmunoassay technique. Fifty minutes after probe insertion, the ventral forearm skin in the area of the dialysis membrane was provoked for 5-15 min with a 25 x 40 mm ice cube covered with plastic foil. In the cold urticaria patients, an up to 80-fold increase of histamine was observed, with peak levels 20-30 min after challenge. Histamine levels then fell to reach "baseline" levels within 50 min. In the healthy subjects, the histamine increase was earlier, less pronounced and of shorter duration. Cutaneous microdialysis and laser Doppler imaging offer new possibilities for the chronological multiparameter assessment of inflammatory skin disorders in vivo.
一种新型的生物分析体内采样技术——皮肤微透析，被用于追踪3例冷荨麻疹患者和2名健康志愿者皮肤组胺释放的时间进程。同时使用激光多普勒灌注成像来监测皮肤循环反应。每隔10分钟采集一次微透析样本，并通过放射免疫分析技术进行分析。在插入探针50分钟后，用一块覆盖有塑料箔的25×40毫米冰块对透析膜区域的前臂腹侧皮肤进行5至15分钟的激发。在冷荨麻疹患者中，观察到组胺增加了80倍，激发后20至30分钟达到峰值水平。然后组胺水平下降，在50分钟内降至“基线”水平。在健康受试者中，组胺增加出现得更早，程度较轻且持续时间较短。皮肤微透析和激光多普勒成像为体内炎症性皮肤病的时间多参数评估提供了新的可能性。
