Anderson C, Andersson T, Wårdell K
Department of Dermatology, University Hospital, Linköping, Sweden.
J Invest Dermatol. 1994 May;102(5):807-11. doi: 10.1111/1523-1747.ep12378630.
Microdialysis makes possible in vivo estimation of endogenous and exogenous substances in the dermal extracellular space. Insertion of the microdialysis probe and its subsequent presence in the skin may affect both the reactivity of the skin test site and the measurement of target substances. Laser Doppler flowmetry is a non-invasive method for estimating cutaneous blood flow. A further development of this technique, laser Doppler perfusion imaging, has been used to study the time course of the circulatory changes caused in the area of microdialysis probe insertion. Laser Doppler perfusion imaging was performed prior to, during, and after microdialysis probe insertion in the skin of the ventral forearm in three subjects. Probe insertion caused an increase in skin blood perfusion in the whole test area. About 15 min after probe insertion, the flare, which is presumed to be of chiefly axon reflex origin, began to subside and the circulatory response could be seen to center around the site of insertion and the tip of the probe. Skin perfusion levels had returned to near normal levels within 60 min. Local anesthesia of the point of guide insertion inhibited the flare, but did not affect circulatory reactivity in the skin nearby. Both microdialysis and laser Doppler perfusion imaging seem to be promising new methods in dermatologic research.
微透析使得在体内估计真皮细胞外间隙中的内源性和外源性物质成为可能。微透析探针的插入及其随后在皮肤中的存在可能会影响皮肤试验部位的反应性以及目标物质的测量。激光多普勒血流仪是一种估计皮肤血流量的非侵入性方法。该技术的进一步发展，即激光多普勒灌注成像，已被用于研究在微透析探针插入区域引起的循环变化的时间进程。在三名受试者的前臂腹侧皮肤中，在微透析探针插入之前、期间和之后进行激光多普勒灌注成像。探针插入导致整个测试区域的皮肤血液灌注增加。探针插入后约15分钟，推测主要起源于轴突反射的潮红开始消退，循环反应可见以插入部位和探针尖端为中心。皮肤灌注水平在60分钟内已恢复到接近正常水平。引导插入点的局部麻醉抑制了潮红，但不影响附近皮肤的循环反应性。微透析和激光多普勒灌注成像似乎都是皮肤病学研究中有前景的新方法。
