Groth L, Jørgensen A, Serup J
Department of Dermatological Research, Leo Pharmaceutical Products, Ballerup, Denmark.
Skin Pharmacol Appl Skin Physiol. 1998 May-Jun;11(3):125-32. doi: 10.1159/000029818.
Microdialysis makes it feasible to study compounds in the extracellular space of cutaneous tissue in vivo. The insertion of the microdialysis probe causes a trauma, which may be anticipated to influence the microdialysis process. The effect of anaesthesia on basal skin blood flow and the trauma in the skin after insertion of a microdialysis probe were investigated. Hairless rats were anaesthetized either with halothane or pentobarbital sodium. Basal skin blood flow and the effect of insertion of a microdialysis probe were measured by laser Doppler perfusion imaging. Trauma-induced histamine release was investigated. Rats anaesthetized with pentobarbital sodium showed a stable skin perfusion in contrast to rats anaesthetized with halothane. A significant increase in blood flow was seen after insertion of the microdialysis probe in the dermis of rats anaesthetized with pentobarbital sodium, while a change in skin blood flow was not observed in rats anaesthetized with halothane. Probe insertion caused histamine release in rats. A minimum equilibration period of 30 min between probe insertion and the start of the experiment is recommended in future studies, allowing immediate events of the trauma to subside and stabilize.
微透析使得在体内研究皮肤组织细胞外空间中的化合物成为可能。微透析探针的插入会造成创伤，预计这可能会影响微透析过程。研究了麻醉对基础皮肤血流以及微透析探针插入后皮肤创伤的影响。用氟烷或戊巴比妥钠对无毛大鼠进行麻醉。通过激光多普勒灌注成像测量基础皮肤血流和微透析探针插入的影响。研究了创伤诱导的组胺释放。与用氟烷麻醉的大鼠相比，用戊巴比妥钠麻醉的大鼠表现出稳定的皮肤灌注。在用戊巴比妥钠麻醉的大鼠真皮中插入微透析探针后，血流显著增加，而在用氟烷麻醉的大鼠中未观察到皮肤血流变化。探针插入导致大鼠组胺释放。在未来的研究中，建议在探针插入和实验开始之间至少有30分钟的平衡期，以使创伤的即时事件消退并稳定下来。
