Field L J, Bruenn J A, Chang T H, Pinhasi O, Koltin Y
Nucleic Acids Res. 1983 May 11;11(9):2765-78. doi: 10.1093/nar/11.9.2765.
UmV is a double-stranded RNA (dsRNA) virus of the corn fungus Ustilago maydis. There are three viral subtypes, P1, P4 and P6, which differ in the specificity of their secreted killer toxins. Each has three size classes of dsRNAs: H (heavy), M (medium), and L (light). We find that, unique among dsRNA viruses, two segments of different size code for the same product--the toxin resistance factor. The smaller dsRNA (L) is homologous to one end of the larger (M), and may have arisen by replication and packaging of a sub-genomic mRNA. We have also compared all the UmV dsRNAs with each other and with the dsRNAs of the similar yeast virus (ScV) by Northern gel and by 3' sequence analysis. Like those of ScV, many of the UmV dsRNAs have one 3' terminus with the sequence UUUUUCAOH or UUUUUCGOH. The H and L dsRNAs of similar size in different viral subtypes are generally related in sequence. The UmV H dsRNAs of different size are not detectably related in sequence.
玉米黑粉菌病毒（UmV）是玉米真菌黑粉菌（Ustilago maydis）的一种双链RNA（dsRNA）病毒。它有三种病毒亚型，即P1、P4和P6，它们分泌的杀伤毒素的特异性有所不同。每种亚型都有三种大小类别的dsRNA：H（重链）、M（中链）和L（轻链）。我们发现，在dsRNA病毒中独一无二的是，两个不同大小的片段编码相同的产物——毒素抗性因子。较小的dsRNA（L）与较大的dsRNA（M）的一端同源，可能是由亚基因组mRNA的复制和包装产生的。我们还通过Northern凝胶电泳和3'序列分析，将所有的UmV dsRNA相互比较，并与类似酵母病毒（ScV）的dsRNA进行了比较。与ScV的dsRNA一样，许多UmV dsRNA的一个3'末端序列为UUUUUCAOH或UUUUUCGOH。不同病毒亚型中大小相似的H和L dsRNA在序列上通常相关。不同大小的UmV H dsRNA在序列上没有可检测到的相关性。
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