Atkinson E F, Cameron L A, Strommer J N
Department of Molecular Biology and Genetics, University of Guelph, Ontario, Canada.
Plant Mol Biol. 1996 Jan;30(2):367-71. doi: 10.1007/BF00020123.
The Adh2 gene from Petunia hybrida has been difficult to clone; exons 1 to 8 were isolated using PCR after unsuccessful screening of three genomic libraries. A combination of inverse and direct PCR strategies has been used to isolate upstream regions of Adh2. Here we report the cloning strategy for the nucleotide sequence of the 5' region of Adh2 from P. hybrida, the locations of the transcriptional start site and putative TATA box, as well as comparative analyses of the upstream regions of petunia Adh2, other Adh genes and other genes induced by hypoxia.
矮牵牛的Adh2基因一直难以克隆；在对三个基因组文库进行筛选未成功后，利用聚合酶链式反应（PCR）分离出了第1至8外显子。反向PCR和直接PCR策略相结合已被用于分离Adh2的上游区域。在此，我们报告了从矮牵牛中克隆Adh2 5'区域核苷酸序列的策略、转录起始位点和假定TATA框的位置，以及矮牵牛Adh2、其他Adh基因和其他缺氧诱导基因上游区域的比较分析。
