Dennis E S, Sachs M M, Gerlach W L, Finnegan E J, Peacock W J
Nucleic Acids Res. 1985 Feb 11;13(3):727-43. doi: 10.1093/nar/13.3.727.
The Adh2 gene of maize has a nucleotide sequence closely related to that of the maize Adh1 gene indicating that the two genes arose from a progenitor gene by a duplication event. The coding regions are 82% conserved at the nucleotide level and 87% conserved at the amino acid level. Each gene has nine introns in identical positions but their nucleotide sequences and lengths differ. Adh2 encodes a single mRNA and has a possible polyadenylation signal, AATAAT, fifteen bases upstream from the polyadenylation site. The Adh2 gene together with the Adh1 gene is induced by anaerobic conditions and under these conditions produces an increased level of mRNA. The 5' untranslated regions of the transcripts of Adh1 (100 bp) and Adh2 (126 bp) have diverged in sequence except for a conserved region which may be important for their anaerobic-specific translation. The 3' and 5' flanking sequences of the two genes are also divergent except for 11 bp of precise homology around the TATA boxes and three other 8 bp sequences further upstream. One of the common 8 bp sequences (CACCTCCC) is in a similar position (-150 bp to -200 bp) in the two genes and may have a regulatory function in gene expression.
玉米的Adh2基因具有与玉米Adh1基因密切相关的核苷酸序列，这表明这两个基因是通过复制事件从一个祖先基因产生的。编码区在核苷酸水平上保守82%，在氨基酸水平上保守87%。每个基因在相同位置有九个内含子，但它们的核苷酸序列和长度不同。Adh2编码一种单一的mRNA，并且在多聚腺苷酸化位点上游15个碱基处有一个可能的多聚腺苷酸化信号AATAAT。Adh2基因与Adh1基因一起在厌氧条件下被诱导，并且在这些条件下产生增加水平的mRNA。Adh1（100bp）和Adh2（126bp）转录本的5'非翻译区除了一个保守区域外，序列已经发生分歧，该保守区域可能对它们的厌氧特异性翻译很重要。除了TATA框周围11bp的精确同源性和上游另外三个8bp序列外，这两个基因的3'和5'侧翼序列也存在分歧。其中一个共同的8bp序列（CACCTCCC）在两个基因中处于相似位置（-150bp至-200bp），可能在基因表达中具有调节功能。
