Choi Y H, Furuse M, Okumura J, Denbow D M
Laboratory of Animal Nutrition, School of Agricultural Sciences, Nagoya University, Japan.
Brain Res. 1995 Nov 13;699(1):161-4. doi: 10.1016/0006-8993(95)01057-3.
Central administration of alpha 2-receptor agonists stimulate food intake in mammalian and avian species. Recently we reported that inhibition of nitric oxide (NO) synthase (NOS) decreased food intake in chickens. In the present study, we investigated whether the increased eating induced by clonidine (Clon), an alpha 2-receptor agonist, is attenuated by NOS inhibition. In the first experiment, four levels (0, 9.4, 18.8 or 37.5 nmol/10 microliters) of Clon were administered into the right lateral ventricle of chickens, and food intake was monitored. Clon increased 30 min-food intake in a dose-dependent manner. In a co-administration study of L-NG-nitro-arginine methyl ester HCl (LNNA), a NOS inhibitor, and Clon, LNNA (0, 1.5, 3.0 or 5.9 mumol) attenuated food intake induced by Clon (37.5 nmol) in a dose-dependent manner. Our results suggest the possibility that NO interacts with adrenergic neurons in the central nervous system to modulate feeding behavior in the chicken.
α2 受体激动剂的中枢给药可刺激哺乳动物和鸟类的食物摄取。最近我们报道，抑制一氧化氮（NO）合酶（NOS）可减少鸡的食物摄取。在本研究中，我们调查了α2 受体激动剂可乐定（Clon）诱导的进食增加是否会被 NOS 抑制所减弱。在第一个实验中，将四个剂量水平（0、9.4、18.8 或 37.5 nmol/10 微升）的 Clon 注入鸡的右侧脑室，并监测食物摄取情况。Clon 以剂量依赖性方式增加 30 分钟的食物摄取量。在 NOS 抑制剂盐酸 L-NG-硝基精氨酸甲酯（LNNA）与 Clon 的联合给药研究中，LNNA（0、1.5、3.0 或 5.9 μmol）以剂量依赖性方式减弱了 Clon（37.5 nmol）诱导的食物摄取。我们的结果提示，NO 可能与中枢神经系统中的肾上腺素能神经元相互作用，以调节鸡的摄食行为。
