人类SIX1基因的克隆及其在14号染色体上的定位。

Cloning of the human SIX1 gene and its assignment to chromosome 14.

作者信息

Boucher C A, Carey N, Edwards Y H, Siciliano M J, Johnson K J

机构信息

Genetics Unit, Department of Anatomy, Charing Cross and Westminster Medical School, Hammersmith, London, W6 8RF, UK.

出版信息

Genomics. 1996 Apr 1;33(1):140-2. doi: 10.1006/geno.1996.0172.

DOI:10.1006/geno.1996.0172

PMID:8617500

Abstract

The recently described murine homeobox genes, Six1 and Six2, which are expressed during development in limb tendons, have also been shown to be expressed in skeletal and smooth muscle, respectively. We have cloned and sequenced a human SIX1 cDNA and shown by Northern blotting that it is expressed in adult skeletal muscle. The cDNA sequence and predicted protein sequence of SIX1 and Six1 are highly homologous, with 98% similarity over the entire predicted amino acid sequence. SIX1 was mapped to human chromosome 14 using a rodent/human somatic cell hybrid panel.

摘要

最近描述的小鼠同源盒基因Six1和Six2在肢体肌腱发育过程中表达，也分别在骨骼肌和平滑肌中表达。我们克隆并测序了人类SIX1 cDNA，通过Northern印迹法表明它在成人骨骼肌中表达。SIX1和Six1的cDNA序列及预测的蛋白质序列高度同源，在整个预测的氨基酸序列上相似度达98%。利用啮齿动物/人类体细胞杂交板将SIX1定位到人类14号染色体上。