Moretti P A, Davidson A J, Baker E, Lilley B, Zon L I, D'Andrea R J
Human Immunology Division and Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Adelaide, South Australia, 5000, Australia.
Genomics. 2001 Aug;76(1-3):21-9. doi: 10.1006/geno.2001.6574.
We have isolated a previously unknown human homeobox-containing cDNA, VENT-like homeobox-2 (VENTX2), using PCR with a bone marrow cDNA library and primers designed from the VENTX1 (alias HPX42) homeobox sequence. Here we describe the molecular cloning, chromosomal localization to 10q26.3, and functional analysis of this gene. The 2.4-kb human VENTX2 cDNA encoded a protein with a predicted molecular weight of 28 kDa containing a homeodomain with 65% identity to the Xenopus laevis ventralizing gene Xvent2B. VENTX2 antisera detected a 28-kDa protein in cells transfected with a VENTX2 expression construct, in a human erythroleukemic cell line and in bone marrow samples obtained from patients in recovery phase after chemotherapy. The similarity of the homeodomains from VENTX2 and the X. laevis Vent gene family places them in the same homeodomain class. Consistent with this structural classification, overexpression of VENTX2 in zebrafish embryos led to anterior truncations and failure to form a notochord, which are characteristics of ventralization.
我们使用聚合酶链反应（PCR），以骨髓cDNA文库为模板，并根据VENTX1（别名HPX42）同源框序列设计引物，分离出了一个先前未知的含人类同源框的cDNA，即VENT样同源框-2（VENTX2）。在此，我们描述了该基因的分子克隆、染色体定位至10q26.3以及功能分析。2.4kb的人类VENTX2 cDNA编码一种预测分子量为28kDa的蛋白质，该蛋白质含有一个同源结构域，与非洲爪蟾腹化基因Xvent2B具有65%的同一性。VENTX2抗血清在转染了VENTX2表达构建体的细胞、人红白血病细胞系以及化疗后处于恢复阶段的患者骨髓样本中检测到一种28kDa的蛋白质。VENTX2和非洲爪蟾Vent基因家族的同源结构域相似性，使它们属于同一同源结构域类别。与这种结构分类一致，VENTX2在斑马鱼胚胎中的过表达导致前部截断和未能形成脊索，这是腹化的特征。
