定向诱变：不通过种系传递分析表型。
Targeted mutagenesis: analysis of phenotype without germ line transmission.
作者信息
Nagy A, Rossant J
机构信息
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
出版信息
J Clin Invest. 1996 Mar 15;97(6):1360-5. doi: 10.1172/JCI118555.
Abstract
The available techniques for directed gene manipulation in the mouse are unprecedented in any multicellular organism and make the mouse an invaluable tool for unraveling all aspects of mammalian biology. To realize fully the potential of these genetic tools requires that phenotypic analysis be efficient, rapid, and complete. Genetic chimeras and mosaics, in which mutant cells are mixed with wild-type cells, can be used to augment standard analysis of intact mutant animals and alleviate the time required and the expense involved in generating and maintaining multiple strains of mutant mice.
摘要
在任何多细胞生物中，现有的小鼠定向基因操作技术都是前所未有的，这使得小鼠成为揭示哺乳动物生物学各个方面的宝贵工具。要充分发挥这些遗传工具的潜力，需要高效、快速且全面的表型分析。遗传嵌合体和镶嵌体，即突变细胞与野生型细胞混合，可以用来加强对完整突变动物的标准分析，并减少生成和维持多株突变小鼠所需的时间和费用。
相似文献
1
Targeted mutagenesis: analysis of phenotype without germ line transmission.定向诱变：不通过种系传递分析表型。
J Clin Invest. 1996 Mar 15;97(6):1360-5. doi: 10.1172/JCI118555.
2
Transgenic lambda medaka as a new model for germ cell mutagenesis.转基因青鳉作为生殖细胞诱变的新模型。
Environ Mol Mutagen. 2008 Apr;49(3):173-84. doi: 10.1002/em.20364.
3
Analysis of mouse eye development with chimeras and mosaics.利用嵌合体和镶嵌体对小鼠眼睛发育进行分析。
Int J Dev Biol. 2004;48(8-9):793-804. doi: 10.1387/ijdb.041885jc.
4
Efficient gene-driven germ-line point mutagenesis of C57BL/6J mice.C57BL/6J小鼠高效基因驱动的种系点突变
BMC Genomics. 2005 Nov 21;6:164. doi: 10.1186/1471-2164-6-164.
5
Production of germ-line chimeras in rainbow trout by blastomere transplantation.通过卵裂球移植在虹鳟鱼中产生种系嵌合体。
Mol Reprod Dev. 2001 Aug;59(4):380-9. doi: 10.1002/mrd.1044.
6
Breaking the species barrier: derivation of germline-competent embryonic stem cells from Mus spretus x C57BL/6 hybrids.突破物种屏障：从家鼠与C57BL/6杂交种中获得具有生殖系能力的胚胎干细胞。
Stem Cells. 2004;22(4):441-7. doi: 10.1634/stemcells.22-4-441.
7
Targeted mutagenesis of the Sap47 gene of Drosophila: flies lacking the synapse associated protein of 47 kDa are viable and fertile.果蝇Sap47基因的靶向诱变：缺乏47 kDa突触相关蛋白的果蝇可存活且可育。
BMC Neurosci. 2004 Apr 29;5:16. doi: 10.1186/1471-2202-5-16.
8
F0 generation mice fully derived from gene-targeted embryonic stem cells allowing immediate phenotypic analyses.完全源自基因靶向胚胎干细胞的F0代小鼠可立即进行表型分析。
Nat Biotechnol. 2007 Jan;25(1):91-9. doi: 10.1038/nbt1263. Epub 2006 Dec 24.
9
No evidence for germ-line transmission following prenatal and early postnatal AAV-mediated gene delivery.没有证据表明产前和产后早期经腺相关病毒介导的基因传递后存在种系传递。
J Gene Med. 2005 May;7(5):630-7. doi: 10.1002/jgm.718.
10
Genetic tools used for cell lineage tracing and gene manipulation in Drosophila germline stem cells.用于果蝇生殖系干细胞中细胞谱系追踪和基因操作的遗传工具。
Methods Mol Biol. 2008;450:61-70. doi: 10.1007/978-1-60327-214-8_4.
引用本文的文献
1
Cre recombinase mediated alterations of the mouse genome using embryonic stem cells.利用胚胎干细胞通过Cre重组酶介导对小鼠基因组进行改造。
Methods Mol Biol. 2008;461:111-32. doi: 10.1007/978-1-60327-483-8_8.
2
Construction of gene-targeting vectors: a rapid Mu in vitro DNA transposition-based strategy generating null, potentially hypomorphic, and conditional alleles.基因靶向载体的构建：一种基于体外快速 Mu 转座的策略，可产生无效、可能的亚效和条件性等位基因。
Transgenic Res. 2001;10(1):69-80. doi: 10.1023/a:1008959231644.
3
Placental cell fates are regulated in vivo by HIF-mediated hypoxia responses.胎盘细胞命运在体内受缺氧诱导因子（HIF）介导的缺氧反应调控。
Genes Dev. 2000 Dec 15;14(24):3191-203. doi: 10.1101/gad.853700.
4
Demystified ... gene knockouts.揭秘……基因敲除。
Mol Pathol. 1999 Jun;52(3):111-6. doi: 10.1136/mp.52.3.111.
5
Targeted null-mutation in the vascular endothelial-cadherin gene impairs the organization of vascular-like structures in embryoid bodies.血管内皮钙黏蛋白基因的靶向无效突变会损害胚状体中血管样结构的组织。
Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6273-8. doi: 10.1073/pnas.94.12.6273.
6
Chimeric mice carrying 'regional' targeted deletion of the angiotensin type 1A receptor gene. Evidence against the role for local angiotensin in the in vivo feedback regulation of renin synthesis in juxtaglomerular cells.携带血管紧张素1A受体基因“区域”靶向缺失的嵌合小鼠。关于局部血管紧张素在球旁细胞肾素合成体内反馈调节中作用的反对证据。
J Clin Invest. 1996 Oct 15;98(8):1867-77. doi: 10.1172/JCI118988.
7
Ligand-activated site-specific recombination in mice.小鼠中的配体激活位点特异性重组
Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10887-90. doi: 10.1073/pnas.93.20.10887.
本文引用的文献
1
The receptor tyrosine kinase TIE is required for integrity and survival of vascular endothelial cells.受体酪氨酸激酶TIE是血管内皮细胞完整性和存活所必需的。
EMBO J. 1995 Dec 1;14(23):5884-91. doi: 10.1002/j.1460-2075.1995.tb00276.x.
2
In vitro differentiation of murine embryonic stem cells. New approaches to old problems.小鼠胚胎干细胞的体外分化。解决老问题的新方法。
J Clin Invest. 1996 Feb 1;97(3):591-5. doi: 10.1172/JCI118454.
3
RAG-2-deficient blastocyst complementation: an assay of gene function in lymphocyte development.RAG-2缺陷型囊胚互补：淋巴细胞发育中基因功能的一种检测方法。
Proc Natl Acad Sci U S A. 1993 May 15;90(10):4528-32. doi: 10.1073/pnas.90.10.4528.
4
Nodal is a novel TGF-beta-like gene expressed in the mouse node during gastrulation.Nodal是一种在原肠胚形成期小鼠原节中表达的新型TGF-β样基因。
Nature. 1993 Feb 11;361(6412):543-7. doi: 10.1038/361543a0.
5
Derivation of completely cell culture-derived mice from early-passage embryonic stem cells.从早期传代胚胎干细胞中获得完全由细胞培养衍生的小鼠。
Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8424-8. doi: 10.1073/pnas.90.18.8424.
6
Essential role of Mash-2 in extraembryonic development.Mash-2在胚外发育中的重要作用。
Nature. 1994 Sep 22;371(6495):333-6. doi: 10.1038/371333a0.
7
An early haematopoietic defect in mice lacking the transcription factor GATA-2.缺乏转录因子GATA-2的小鼠早期造血缺陷。
Nature. 1994 Sep 15;371(6494):221-6. doi: 10.1038/371221a0.
8
The winged-helix transcription factor HNF-3 beta is required for notochord development in the mouse embryo.有翼螺旋转录因子HNF-3β是小鼠胚胎脊索发育所必需的。
Cell. 1994 Aug 26;78(4):575-88. doi: 10.1016/0092-8674(94)90523-1.
9
HNF-3 beta is essential for node and notochord formation in mouse development.肝细胞核因子3β对于小鼠发育过程中节点和脊索的形成至关重要。
Cell. 1994 Aug 26;78(4):561-74. doi: 10.1016/0092-8674(94)90522-3.
10
Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting.利用细胞类型特异性基因靶向技术删除T细胞中的DNA聚合酶β基因片段。
Science. 1994 Jul 1;265(5168):103-6. doi: 10.1126/science.8016642.
Zotero 插件