Gu H, Marth J D, Orban P C, Mossmann H, Rajewsky K
Institute for Genetics, University of Cologne, Germany.
Science. 1994 Jul 1;265(5168):103-6. doi: 10.1126/science.8016642.
Deletion of the promoter and the first exon of the DNA polymerase beta gene (pol beta) in the mouse germ line results in a lethal phenotype. With the use of the bacteriophage-derived, site-specific recombinase Cre in a transgenic approach, the same mutation can be selectively introduced into a particular cellular compartment-in this case, T cells. The impact of the mutation on those cells can then be analyzed because the mutant animals are viable.
在小鼠生殖系中删除DNA聚合酶β基因（polβ）的启动子和第一个外显子会导致致死表型。通过在转基因方法中使用噬菌体衍生的位点特异性重组酶Cre，可以将相同的突变选择性地引入特定的细胞区室——在这种情况下是T细胞。由于突变动物是存活的，因此可以分析该突变对这些细胞的影响。
