Hardas B D, Zhao X, Zhang J, Longqing X, Stoll S, Elder J T
Department of Dermatology, University of Michigan, Ann Arbor, USA.
J Invest Dermatol. 1996 Apr;106(4):753-8. doi: 10.1111/1523-1747.ep12345807.
Psoriasin is an abundant low molecular weight protein in keratinocytes from psoriatic lesions. Because of similarities in gene structure and expression to other genes on human chromosomal band 1q21, we hypothesized that psoriasin might also map to this region. To test this hypothesis, we identified and used a genomic lambda clone (lambda 9.2) as a probe for fluorescent in situ hybridization. lambda 9.2 detected the 1q21 region in 81% of 52 chromosomes 1 examined, although it also hybridized to acrocentric chromosomes. lambda 9.2 DNA yielded polymerase chain reaction amplification of 121-bp sequence colinear with psoriasin cDNA, as did genomic DNA from hybrid cell lines containing all or part of chromosome 1. The psoriasin gene was present on a 380-kb yeast artificial chromosome clone that was previously mapped to 1q21 and shown to contain calcyclin; here it is also shown to contain MRP8 and CaN19. Psoriasin and several other tightly linked 1q21 genes were markedly overexpressed in psoriatic lesions, suggesting a role for these clustered genes in the regulation of epidermal proliferation.
银屑素是银屑病皮损角质形成细胞中一种丰富的低分子量蛋白质。由于其基因结构和表达与人类染色体1q21上的其他基因相似，我们推测银屑素可能也定位于该区域。为了验证这一假设，我们鉴定并使用了一个基因组λ克隆（λ9.2）作为荧光原位杂交的探针。λ9.2在检测的52条1号染色体中有81%检测到1q21区域，尽管它也与近端着丝粒染色体杂交。λ9.2 DNA产生了与银屑素cDNA共线的121 bp序列的聚合酶链反应扩增，含有全部或部分1号染色体的杂交细胞系的基因组DNA也如此。银屑素基因存在于一个380 kb的酵母人工染色体克隆上，该克隆先前已定位到1q21并显示含有钙周期蛋白；此处还显示它含有MRP8和CaN19。银屑素和其他几个紧密连锁的1q21基因在银屑病皮损中明显过表达，提示这些成簇基因在表皮增殖调控中起作用。
