Sundaram S G, Milner J A
Department of Nutrition, The Pennsylvania State University, University Park, 16802, USA.
J Nutr. 1996 May;126(5):1355-61. doi: 10.1093/jn/126.5.1355.
The present studies examined the anti-proliferative effects of diallyl disulfide (DADS) on the growth of human colon tumor cell line, HCT-15, xenografts in 6-wk-old female NCr nu/nu mice with an initial body weight of 20-22 g. Intraperitoneal injection of 1 mg DADS thrice weekly reduced tumor volume by 69% (P < 0.05) without apparent ill consequences such as altered growth of the host. Providing this quantity of DADS intragastrically also inhibited growth of the HCT-15 tumor. At equivalent DADS dosages, intraperitoneal treatment was proportionately more effective (P < 0.05) in reducing tumor growth than gastric intubation. Tumor inhibition caused by DADS (0.5 mg thrice weekly) was similar to that occurring with 5-fluorouracil (5-FU) treatment (0.5 mg thrice weekly). Combining DADS and 5-FU was no more effective in inhibiting tumor growth than using either compound alone. However, concurrent DADS treatment significantly (P < 0.05) inhibited the depression in leukocyte counts and spleen weight and prevented the elevated plasma urea caused by 5-FU treatment. These data suggest that DADS, a constituent of garlic oil, reduces the toxicity of 5-FU and is an effective antitumorigenic agent against xenografts resulting from an established human colon tumor cell line.
本研究检测了二烯丙基二硫醚（DADS）对人结肠肿瘤细胞系HCT - 15在6周龄、初始体重20 - 22 g的雌性NCr裸鼠体内异种移植瘤生长的抗增殖作用。每周三次腹腔注射1 mg DADS可使肿瘤体积减小69%（P < 0.05），且无明显不良后果，如宿主生长改变。胃内给予此剂量的DADS也可抑制HCT - 15肿瘤的生长。在等效的DADS剂量下，腹腔给药在减少肿瘤生长方面比胃插管更有效（P < 0.05）。DADS（每周三次，每次0.5 mg）引起的肿瘤抑制作用与5 - 氟尿嘧啶（5 - FU）治疗（每周三次，每次0.5 mg）相似。联合使用DADS和5 - FU在抑制肿瘤生长方面并不比单独使用任何一种化合物更有效。然而，同时给予DADS治疗可显著（P < 0.05）抑制5 - FU治疗引起的白细胞计数降低和脾脏重量减轻，并防止血浆尿素升高。这些数据表明，大蒜油的成分DADS可降低5 - FU的毒性，是一种针对已建立的人结肠肿瘤细胞系异种移植瘤有效的抗肿瘤剂。
