Sundaram S G, Milner J A
Department of Nutrition, Pennsylvania State University, University Park, USA.
Carcinogenesis. 1996 Apr;17(4):669-73. doi: 10.1093/carcin/17.4.669.
The present studies compared the effects of various oil-soluble compounds containing allyl and disulfide groups on the proliferation of cultured human colon tumor cells (HCT-15). Diallyl disulfide (DADS) was more effective in inhibiting the growth of HCT-15 cells than isomolar concentrations of S-allyl cysteine, dipropyl disulfide (DPDS), allyl chloride, allyl glycidyl ether and allyl alcohol. These studies clearly demonstrate the importance of both the diallyl and the disulfide groups in DADS. Treatment of HCT-15 cells with 100 microM DADS increased the intracellular calcium levels by 40%, while DPDS caused only a 12% increase in intracellular calcium. Exposure to 100 microM DADS or more, but not DPDS, caused the cells to undergo apoptosis as determined by morphological changes and DNA fragmentation. A positive correlation (r=0.944) was found between DADS-induced DNA fragmentation and its ability to increase intracellular free calcium levels. The widespread effectiveness of DADS was evident by its ability to inhibit the growth of human colon (HCT-15), skin (SK MEL-2) and lung (A549) tumor cell lines.
本研究比较了各种含有烯丙基和二硫键基团的油溶性化合物对培养的人结肠肿瘤细胞（HCT - 15）增殖的影响。与等摩尔浓度的S - 烯丙基半胱氨酸、二丙基二硫（DPDS）、烯丙基氯、烯丙基缩水甘油醚和烯丙醇相比，二烯丙基二硫（DADS）在抑制HCT - 15细胞生长方面更有效。这些研究清楚地证明了DADS中二烯丙基和二硫键基团的重要性。用100微摩尔/升的DADS处理HCT - 15细胞可使细胞内钙水平增加40%，而DPDS仅使细胞内钙增加12%。通过形态学变化和DNA片段化测定，暴露于100微摩尔/升或更高浓度的DADS，但不包括DPDS，会导致细胞发生凋亡。在DADS诱导的DNA片段化与其增加细胞内游离钙水平的能力之间发现了正相关（r = 0.944）。DADS能够抑制人结肠（HCT - 15）、皮肤（SK MEL - 2）和肺（A549）肿瘤细胞系的生长，这表明其具有广泛的有效性。
