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Mammalian phospholipase D: activation by ammonium sulfate and nucleotides.哺乳动物磷脂酶D：硫酸铵和核苷酸的激活作用。
Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12319-22. doi: 10.1073/pnas.92.26.12319.
2
Guanine-nucleotide- and adenine-nucleotide-dependent regulation of phospholipase D in electropermeabilized HL-60 granulocytes.电通透HL-60粒细胞中鸟嘌呤核苷酸和腺嘌呤核苷酸对磷脂酶D的依赖性调节
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3
Activation of phospholipase D by ADP-ribosylation factor in rat submandibular acinar cells.ADP-核糖基化因子对大鼠下颌下腺腺泡细胞中磷脂酶D的激活作用。
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4
Phospholipase D activation in a cell-free system from human neutrophils by phorbol 12-myristate 13-acetate and guanosine 5'-O-(3-thiotriphosphate). Activation is calcium dependent and requires protein factors in both the plasma membrane and cytosol.佛波醇12-肉豆蔻酸酯13-乙酸酯和鸟苷5'-O-(3-硫代三磷酸)在人中性粒细胞无细胞体系中激活磷脂酶D。激活依赖于钙，且需要质膜和胞质溶胶中的蛋白质因子。
J Biol Chem. 1991 Sep 15;266(26):17236-42.
5
Phorbol ester treatment of intact rabbit platelets greatly enhances both the basal and guanosine 5'-[gamma-thio]triphosphate-stimulated phospholipase D activities of isolated platelet membranes. Physiological activation of phospholipase D may be secondary to activation of phospholipase C.佛波酯处理完整的兔血小板可显著增强分离的血小板膜的基础磷脂酶D活性以及鸟苷5'-[γ-硫代]三磷酸刺激的磷脂酶D活性。磷脂酶D的生理激活可能继发于磷脂酶C的激活。
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6
Requirement of adenosine 5'-triphosphate and Ca2+ for guanosine 5'-triphosphate-binding protein-mediated phospholipase D activation in rat pheochromocytoma PC12 cells.
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7
Guanosine 5'-[gamma-thio]triphosphate induces membrane localization of cytosol-independent phospholipase D activity in a cell-free system from U937 promonocytic leucocytes.鸟苷5'-[γ-硫代]三磷酸在来自U937前单核细胞的无细胞体系中诱导不依赖胞质溶胶的磷脂酶D活性的膜定位。
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8
Activation of phospholipase D by guanosine 5'[gamma-thio]triphosphate and AlF4- in bovine corneal epithelial cells.鸟苷 5'-[γ-硫代]三磷酸和 AlF4- 在牛角膜上皮细胞中对磷脂酶 D 的激活作用。
Curr Eye Res. 1994 Mar;13(3):231-8. doi: 10.3109/02713689408995782.
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Mammalian phospholipase D: phosphatidylethanolamine as an essential component.哺乳动物磷脂酶D：磷脂酰乙醇胺作为必需成分。
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Phospholipase D: a downstream effector of ARF in granulocytes.磷脂酶D：粒细胞中ARF的下游效应分子。
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Biochem J. 2000 Dec 1;352 Pt 2(Pt 2):491-9. doi: 10.1042/0264-6021:3520491.
6
Effect of dietary lipids on phospholipase D activity in rat brain.
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7
Requirement of GM2 ganglioside activator for phospholipase D activation.GM2神经节苷脂激活剂对磷脂酶D激活的需求。
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8
Different mechanisms regulate phosphatidylserine synthesis in rat cerebral cortex.不同的机制调节大鼠大脑皮层中磷脂酰丝氨酸的合成。
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Didecanoyl phosphatidylcholine is a superior substrate for assaying mammalian phospholipase D.二癸酰磷脂酰胆碱是用于测定哺乳动物磷脂酶D的优质底物。
Biochem J. 1996 Nov 1;319 ( Pt 3)(Pt 3):861-4. doi: 10.1042/bj3190861.
10
Mammalian phospholipase D: phosphatidylethanolamine as an essential component.哺乳动物磷脂酶D：磷脂酰乙醇胺作为必需成分。
Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):4300-4. doi: 10.1073/pnas.93.9.4300.

本文引用的文献

1
Phosphatidic acid directly activates endothelial cell protein kinase C.磷脂酸直接激活内皮细胞蛋白激酶C。
Biochem Biophys Res Commun. 1993 Feb 26;191(1):134-41. doi: 10.1006/bbrc.1993.1194.
2
Neutrophil phospholipase D is activated by a membrane-associated Rho family small molecular weight GTP-binding protein.中性粒细胞磷脂酶D由一种膜相关的Rho家族小分子重量GTP结合蛋白激活。
J Biol Chem. 1993 Oct 15;268(29):21509-12.
3
Phospholipase D: a downstream effector of ARF in granulocytes.磷脂酶D：粒细胞中ARF的下游效应分子。
Science. 1994 Jan 28;263(5146):523-6. doi: 10.1126/science.8290961.
4
ADP-ribosylation factor, a small GTP-dependent regulatory protein, stimulates phospholipase D activity.ADP核糖基化因子是一种依赖鸟苷三磷酸的小调节蛋白，可刺激磷脂酶D的活性。
Cell. 1993 Dec 17;75(6):1137-44. doi: 10.1016/0092-8674(93)90323-i.
5
Lipid second messengers.脂质第二信使
Cell. 1994 May 6;77(3):329-34. doi: 10.1016/0092-8674(94)90148-1.
6
A role for phospholipase D in control of mitogenesis.
Trends Pharmacol Sci. 1994 Feb;15(2):57-62. doi: 10.1016/0165-6147(94)90111-2.
7
Purification and characterization of phosphatidylcholine phospholipase D from pig lung.猪肺磷脂酰胆碱磷脂酶D的纯化与特性分析
J Biol Chem. 1994 Dec 9;269(49):31207-13.
8
Lipid mediators and protein kinase C activation for the intracellular signaling network.
J Biochem. 1994 Jun;115(6):1029-34. doi: 10.1093/oxfordjournals.jbchem.a124451.
9
Activation of rat brain phospholipase D by ADP-ribosylation factors 1,5, and 6: separation of ADP-ribosylation factor-dependent and oleate-dependent enzymes.ADP-核糖基化因子1、5和6对大鼠脑磷脂酶D的激活作用：ADP-核糖基化因子依赖性酶和油酸依赖性酶的分离
Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11718-22. doi: 10.1073/pnas.91.24.11718.
10
Activation of rat liver phospholipase D by the small GTP-binding protein RhoA.小GTP结合蛋白RhoA对大鼠肝脏磷脂酶D的激活作用。
J Biol Chem. 1994 Oct 21;269(42):25951-4.

哺乳动物磷脂酶D：硫酸铵和核苷酸的激活作用。

Mammalian phospholipase D: activation by ammonium sulfate and nucleotides.

作者信息

Nakamura S, Shimooku K, Akisue T, Jinnai H, Hitomi T, Kiyohara Y, Ogino C, Yoshida K, Nishizuka Y

机构信息

Department of Biochemistry, Kobe University School of Medicine, Japan.

出版信息

Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12319-22. doi: 10.1073/pnas.92.26.12319.

DOI:10.1073/pnas.92.26.12319
PMID:8618893
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC40348/
Abstract

Phospholipase D (PLD) associated with the rat kidney membrane was activated by guanine 5'-[gamma-thio]triphosphate and a cytosol fraction that contained ADP-ribosylation factor. When assayed by measuring the phosphatidyl transfer reaction to ethanol with exogenously added radioactive phosphatidylcholine as substrate, the PLD required a high concentration (1.6 M) of ammonium sulfate to exhibit high enzymatic activity. Other salts examined were far less effective or practically inactive, and this dramatic action of ammonium sulfate is not simply due to such high ionic strength. Addition of ATP but not of nonhydrolyzable ATP analogue adenosine 5'-[beta, gamma-imido]diphosphate further enhanced the PLD activation approximately equal to 2- to 3-fold. This enhancement by ATP needed cytosol, implying a role of protein phosphorylation. A survey of PLD activity in rat tissues revealed that, unlike in previous observations reported thus far, PLD was most abundant in membrane fractions of kidney, spleen, and liver in this order, and the enzymatic activity in brain and lung was low.

摘要

与大鼠肾膜相关的磷脂酶D（PLD）被鸟嘌呤5'-[γ-硫代]三磷酸和含有ADP-核糖基化因子的胞质溶胶部分激活。当以外源添加的放射性磷脂酰胆碱为底物，通过测量向乙醇的磷脂酰转移反应来测定时，PLD需要高浓度（1.6 M）的硫酸铵才能表现出高酶活性。所检测的其他盐效果要差得多或实际上无活性，硫酸铵的这种显著作用并非仅仅归因于如此高的离子强度。添加ATP而非不可水解的ATP类似物腺苷5'-[β，γ-亚氨基]二磷酸可进一步增强PLD的激活，增强幅度约为2至3倍。ATP的这种增强作用需要胞质溶胶，这意味着蛋白质磷酸化起作用。对大鼠组织中PLD活性的调查显示，与迄今为止报道的先前观察结果不同，PLD在肾、脾和肝的膜部分中含量最丰富，顺序依次为肾、脾、肝，而脑和肺中的酶活性较低。