Adone R, Ciuchini F, Bianchi R, Piccininno G, Pistoia C
Istituto Superiore di Sanità. Laboratorio di Medicina Veterinaria, Roma, Italia.
Vet Res Commun. 1995;19(6):457-61. doi: 10.1007/BF01839333.
The protective anti-beta abortus monoclonal antibody ISS/32 (Ab1) was used as an immunogen to induce anti-idiotypic antibodies (Ab2) in rabbits. The purpose was to produce and characterize anti-idiotypic antibodies that share conformational similarity with the corresponding bacterial epitope recognized by Ab1. The rabbit anti-IdAb so induced was isolated and affinity-purified. Its specificity for the paratope of Ab1 was determined by evaluating its ability to compete with B. abortus for binding to Ab1 in a competitive ELISA assay. The anti-idiotypic ISS/32 antibodies were able to compete with B. abortus for binding to Ab1 in a dose-dependent manner. Hence, the data indicated that the rabbit anti-Id ISS/32 antibodies reacted with or near the antigen-binding site of Ab1.
保护性抗流产布鲁氏菌单克隆抗体ISS/32（Ab1）被用作免疫原，以在兔体内诱导抗独特型抗体（Ab2）。目的是产生并鉴定与Ab1识别的相应细菌表位具有构象相似性的抗独特型抗体。如此诱导产生的兔抗独特型抗体（IdAb）被分离并进行亲和纯化。通过在竞争性酶联免疫吸附测定（ELISA）中评估其与流产布鲁氏菌竞争结合Ab1的能力，确定其对Ab1互补位的特异性。抗独特型ISS/32抗体能够以剂量依赖的方式与流产布鲁氏菌竞争结合Ab1。因此，数据表明兔抗Id ISS/32抗体与Ab1的抗原结合位点或其附近发生反应。
