van Cuyck-Gandre H, Caudill J D, Zhang H Y, Longer C F, Molinie C, Roue R, Deloince R, Coursaget P, Mamouth N N, Buisson Y
Centre de Recherche du Service de Sante des Armees, La Tronche, France.
Am J Trop Med Hyg. 1996 Feb;54(2):134-5. doi: 10.4269/ajtmh.1996.54.134.
Epidemics of enterically-transmitted non-A, non-B hepatitis were described in 1983-1984 involving French soldiers in Chad and in 1979-1980 in residents of Algeria. Hepatitis E virus (HEV) was subsequently implicated by serology. In this study, the presence of HEV in patient stool specimens from both outbreaks and from sporadic cases in residents of Chad (1994) was documented. This virus was detected in fecal suspensions by antibody capture of the virus and reverse transcriptase-polymerase chain reaction amplification of the viral RNA in the 3' end of open reading frame 2. Two of five epidemic cases from Chad (1983-1984) were positive, as well as one of five sporadic cases from Chad (1994), and two of three epidemic cases from Algeria (1979-1980). Of these 13 patients, 12 had detectable anti-HEV IgG in their serum. These results confirmed that HEV was the cause of hepatitis in at least five of these 13 patients.
1983 - 1984年，在乍得的法国士兵中以及1979 - 1980年在阿尔及利亚居民中出现了经肠道传播的非甲非乙型肝炎疫情。随后通过血清学检测发现戊型肝炎病毒（HEV）与此有关。在本研究中，记录了来自这两次疫情以及乍得居民散发病例（1994年）患者粪便标本中HEV的存在情况。通过病毒抗体捕获以及对开放阅读框2 3'端病毒RNA进行逆转录 - 聚合酶链反应扩增，在粪便悬液中检测到了这种病毒。来自乍得（1983 - 1984年）的5例疫情病例中有2例呈阳性，乍得（1994年）的5例散发病例中有1例呈阳性，阿尔及利亚（1979 - 1980年）的3例疫情病例中有2例呈阳性。在这13名患者中，有12名患者血清中可检测到抗HEV IgG。这些结果证实，在这13名患者中至少有5名患者的肝炎病因是HEV。
