Jones P A, Baker M S, Bertram J S, Benedict W F
Cancer Res. 1977 Jul;37(7 Pt 1):2214-7.
1-beta-Darabinofuranosylcytosine at concentrations ranging from 10(-3) to 10(-6) M induces oncogenic transformation in the C3H/10T1/2 clone 8 mouse embryo cell line. Cell lines derived from type III transformed foci grew in soft agarose and produced tumors in immunosuppressed syngeneic mice. With cells synchronized by postconfluent inhibition of growth or isoleucine deprivation, transformation was cell cycle dependent. Maximal transformation was seen in cells treated when in S phase, although some transformation was seen in cells treated in G1 phase of the cell cycle.
浓度范围为10⁻³至10⁻⁶ M的1-β-D-阿拉伯呋喃糖基胞嘧啶可诱导C3H/10T1/2克隆8小鼠胚胎细胞系发生致癌转化。源自III型转化灶的细胞系可在软琼脂中生长，并在免疫抑制的同基因小鼠中产生肿瘤。通过汇合后生长抑制或异亮氨酸剥夺使细胞同步化，转化呈细胞周期依赖性。在S期处理的细胞中可见最大程度的转化，尽管在细胞周期的G1期处理的细胞中也可见一些转化。
