Pereira A S, Franco R, Feio M J, Pinto C, Lampreia J, Reis M A, Calvete J, Moura I, Beech I, Lino A R, Moura J J
Departmento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Portugal.
Biochem Biophys Res Commun. 1996 Apr 16;221(2):414-21. doi: 10.1006/bbrc.1996.0610.
This communication reports the isolation, purification and characterization of key enzymes involved in dissimilatory sulfate reduction of a sulfate reducing bacterium classified as Desulfovibrio desulfuricans subspecies desulfuricans New Jersey (NCIMB 8313) (Ddd NJ). The chosen strain, originally recovered from a corroding cast iron heat exchanger, was grown in large scale batch cultures. Physico-chemical and spectroscopic studies of the purified enzymes were carried out. These analyses revealed a high degree of similarity between proteins isolated from the DddNJ strain and the homologous proteins obtained from Desulfomicrobium baculatus Norway 4. In view of the results obtained, taxonomic reclassification of Desulfovibrio desulfuricans subspecies desulfuricans New Jersey (NCIMB 8313) into Desulfomicrobium baculatus (New Jersey) is proposed.
本通讯报道了对一种被归类为脱硫弧菌脱硫亚种新泽西菌株（NCIMB 8313）（Ddd NJ）的硫酸盐还原细菌中参与异化硫酸盐还原的关键酶的分离、纯化及特性鉴定。所选菌株最初从一个正在腐蚀的铸铁热交换器中分离得到，在大规模分批培养中生长。对纯化后的酶进行了物理化学和光谱学研究。这些分析表明，从DddNJ菌株中分离出的蛋白质与从挪威杆状脱硫微菌4中获得的同源蛋白质具有高度相似性。鉴于所获得的结果，建议将脱硫弧菌脱硫亚种新泽西菌株（NCIMB 8313）重新分类为杆状脱硫微菌（新泽西）。
