Kummer U, Valeur K R, Baier G, Wegmann K, Olsen L F
Institute for Chemical Plant Physiology, University of Tübingen, Germany.
Biochim Biophys Acta. 1996 Apr 17;1289(3):397-403. doi: 10.1016/0304-4165(95)00176-x.
The nonlinear behavior of the peroxidase-oxidase reaction was studied using structurally different peroxidases. For the first time sustained oscillations with peroxidases other than horseradish peroxidase in a single-enzyme system were observed. All peroxidases that showed significant oxidase activity were able to generate sustained oscillations. When adjusting the overall reaction rate, either of the two modifiers 2,4-dichlorophenol or Methylene blue could be omitted from the reaction. Due to the observation of different enzyme intermediates when using different peroxidases, we conclude that the mechanisms responsible for oscillatory kinetics may vary from one peroxidase to the other.
使用结构不同的过氧化物酶研究了过氧化物酶-氧化酶反应的非线性行为。首次在单酶体系中观察到除辣根过氧化物酶外的其他过氧化物酶产生持续振荡。所有表现出显著氧化酶活性的过氧化物酶都能够产生持续振荡。当调节总反应速率时，反应中两种调节剂2,4-二氯苯酚或亚甲蓝中的任何一种都可以省略。由于使用不同过氧化物酶时观察到不同的酶中间体，我们得出结论，负责振荡动力学的机制可能因过氧化物酶不同而有所差异。
