Gunji Y, Tagawa M, Matsubara H, Takenaga K, Shimada H, Kondo F, Suzuki T, Nakajima K, Sugaya M, Asano T, Ochiai T, Isono K, Horitsu K, Kageyama H, Nakamura Y, Sakiyama S
Department of Surgery (II), School of Medicine, Chiba University, Japan.
Cancer Lett. 1996 Mar 29;101(2):257-61. doi: 10.1016/0304-3835(96)04141-9.
Murine colon carcinoma cells which secrete several kinds of cytokine after retroviral transduction with corresponding genes, were examined for their antitumor effects in syngeneic mice. The mice inoculated with granulocyte macrophage-colony stimulating factor (GM-CSF) producer cells showed not only prolonged survival but also reduced tumorigenicity. The antitumor effect caused by the expression of interleukin-4 was less than that of GM-CSF, and interleukin-6 producer cells did not show any effects on the survival of the host animals. Histological examination of the GM-CSF-producing tumor revealed predominant infiltration of neutrophils and necrotic change of the tumor. The present study indicates the feasibility of cancer gene therapy with the expression of GM-CSF gene in tumor cells.
用相应基因进行逆转录病毒转导后分泌多种细胞因子的小鼠结肠癌细胞，在同基因小鼠中检测其抗肿瘤作用。接种粒细胞巨噬细胞集落刺激因子（GM-CSF）产生细胞的小鼠不仅存活期延长，而且致瘤性降低。白细胞介素-4表达所引起的抗肿瘤作用小于GM-CSF，白细胞介素-6产生细胞对宿主动物的存活没有任何影响。对产生GM-CSF的肿瘤进行组织学检查发现，肿瘤有大量中性粒细胞浸润和坏死改变。本研究表明在肿瘤细胞中表达GM-CSF基因进行癌症基因治疗的可行性。
