Haseley S R, Wilkinson S G
School of Chemistry, University of Hull, England.
Eur J Biochem. 1996 Apr 1;237(1):229-33. doi: 10.1111/j.1432-1033.1996.0229n.x.
A polysaccharide containing 2-acetamido-2-deoxy-D-glucose (GlcNAc), 2-acetamido-2-deoxy-L-fucose (FucNAc), and 2-acetamido-2-deoxy-D-galacturonic acid (GalNAcA) was isolated from an aqueous phenol extract of lipid-free, isolated cell walls of the reference strain for Acinetobacter baumannii serogroup O5, by mild acid hydrolysis of the extract and chromatography of the water-soluble products on Sephadex G-50. By means of NMR studies, methylation analysis, carboxyl reduction and chemical degradations, the repeating unit of the polymer was identified as a branched tetrasaccharide of the structure shown. The serologically active polymer is believed to correspond to the side chain of the O5 lipopolysaccharide: [table: see text]
从鲍曼不动杆菌O5血清群参考菌株无脂分离细胞壁的水苯酚提取物中，通过温和酸水解提取物并将水溶性产物在葡聚糖G - 50上进行色谱分离，分离出一种含有2 - 乙酰氨基 - 2 - 脱氧 - D - 葡萄糖（GlcNAc）、2 - 乙酰氨基 - 2 - 脱氧 - L - 岩藻糖（FucNAc）和2 - 乙酰氨基 - 2 - 脱氧 - D - 半乳糖醛酸（GalNAcA）的多糖。通过核磁共振研究、甲基化分析、羧基还原和化学降解，确定该聚合物的重复单元为所示结构的支链四糖。据信，具有血清学活性的聚合物对应于O5脂多糖的侧链：[表格：见正文]
