Haseley S R, Wilkinson S G
School of Chemistry, University of Hull, UK.
Eur J Biochem. 1996 Apr 1;237(1):266-71. doi: 10.1111/j.1432-1033.1996.0266n.x.
A major polysaccharide containing D-galactose, D-glucose and 2-acetamido-2-deoxy-D-galactose was obtained after mild acid hydrolysis of the water-soluble material released by treatment of cell walls from Acinetobacter baumannii strain O11 with hot, aqueous phenol. By means of NMR studies, Smith degradation and N-deacetylation/deamination, the repeating unit of the polymer was identified as a branched pentasaccharide of the structure shown. Also present was a minor polymer containing glucose, 2-acetamido-2-deoxyglucose-and 2-acetamido-2-deoxygalactose, the structure of which was not elucidated. On serological testing, the polymeric material was shown to correspond to the O-antigenic moiety of the parent extract (assumed to be lipopolysaccharide) and circumstantial evidence indicated that O11 specificity was conferred by the major polymer. [formula: see text]
用热的苯酚水溶液处理鲍曼不动杆菌O11菌株的细胞壁后，释放出的水溶性物质经温和酸水解，得到了一种主要的多糖，其含有D-半乳糖、D-葡萄糖和2-乙酰氨基-2-脱氧-D-半乳糖。通过核磁共振研究、史密斯降解和N-脱乙酰化/脱氨作用，确定该聚合物的重复单元为所示结构的支链五糖。还存在一种次要的聚合物，其含有葡萄糖、2-乙酰氨基-2-脱氧葡萄糖和2-乙酰氨基-2-脱氧半乳糖，其结构尚未阐明。血清学检测表明，该聚合物质与亲本提取物（假定为脂多糖）的O抗原部分相对应，间接证据表明O11特异性由主要聚合物赋予。[化学式：见正文]
