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从鲍曼不动杆菌(DNA群2)菌株9的酚溶性脂多糖中分离出的O抗原的结构研究。

Structural studies of the O-antigen isolated from the phenol-soluble lipopolysaccharide of Acinetobacter baumannii (DNA group 2) strain 9.

作者信息

Haseley S R, Holst O, Brade H

机构信息

Division of Medical and Biochemical Microbiology, Research Centre Borstel, Centre for Medicine and Biosciences, Germany.

出版信息

Eur J Biochem. 1998 Jan 15;251(1-2):189-94. doi: 10.1046/j.1432-1327.1998.2510189.x.

DOI:10.1046/j.1432-1327.1998.2510189.x
PMID:9492283
Abstract

A polysaccharide containing D-GalNAc, D-Glc and 4-acetamido-4,6-dideoxy-D-glucose (Qui4NAc) was isolated from the phenol-soluble lipopolysaccharide originating from Acinetobacter baumannii strain 9. The structure of the repeating unit was shown by means of monosaccharide analyses, Smith-degradation, partial acid hydrolysis, mass spectrometry, and NMR spectroscopy to be a branched pentasaccharide, in which the tetrasaccharide backbone is built from amino sugars only. [structure: see text] The polysaccharide was identified by serological and western blot analyses as the O-antigen of the lipopolysaccharide.

摘要

从鲍曼不动杆菌9型菌株的酚溶性脂多糖中分离出一种含有D-氨基半乳糖、D-葡萄糖和4-乙酰氨基-4,6-二脱氧-D-葡萄糖(Qui4NAc)的多糖。通过单糖分析、史密斯降解、部分酸水解、质谱和核磁共振光谱等方法表明,重复单元的结构是一种支链五糖,其中四糖主链仅由氨基糖构成。[结构:见正文] 通过血清学和蛋白质印迹分析确定该多糖为脂多糖的O抗原。

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