一种检测人精子细胞毒性抗体的放射微量测定法。用氚标记的放线菌素D进行定量分析。
A radiomicroassay for cytotoxic antibody to human spermatozoa. Quantification by tritiated actinomycin d.
作者信息
Sung J S, Shizuya H, Black D D, Mumford D M
出版信息
Clin Exp Immunol. 1977 Mar;27(3):469-77.
Abstract
A radiomicroassay for titration of spermocytotoxic antibody is described. The assay used [3H]AACTINOMYCIN D ([3H]Act D) to label damaged spermatozoa in a fashion analogous to penetration by vital dye. Optimal conditions for and some kinetics of the assay are presented. The assay is sensitive, reliable, simple to perform and uses only small amounts of serum and spermatozoa. Applied to sperm antibody positive human postvasectomy sera, the assay compared favourably in sensitivity eith vital dye microscopic observations and with parallel titration by the Isojima's immobilization tests.
摘要
本文描述了一种用于滴定精子细胞毒性抗体的放射微分析法。该分析法使用[3H]放线菌素D（[3H]Act D）以类似于活体染料穿透的方式标记受损精子。文中给出了该分析法的最佳条件及一些动力学数据。该分析法灵敏、可靠、操作简单，且仅需少量血清和精子。将其应用于输精管切除术后精子抗体阳性的人血清时，该分析法在灵敏度方面与活体染料显微镜观察法以及与稻岛固定试验的平行滴定法相比具有优势。
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A radiomicroassay for cytotoxic antibody to human spermatozoa. Quantification by tritiated actinomycin d.一种检测人精子细胞毒性抗体的放射微量测定法。用氚标记的放线菌素D进行定量分析。
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