Coulie P G, Brichard V, Van Pel A, Wölfel T, Schneider J, Traversari C, Mattei S, De Plaen E, Lurquin C, Szikora J P, Renauld J C, Boon T
Ludwig Institute for Cancer Research, Brussels, Belgium.
J Exp Med. 1994 Jul 1;180(1):35-42. doi: 10.1084/jem.180.1.35.
It has been reported previously that antitumor cytolytic T lymphocyte (CTL) clones can be isolated from blood lymphocytes of HLA-A2 melanoma patients, after stimulation in vitro with autologous tumor cells, and that some of these CTL clones lyse most HLA-A2 melanomas. A first antigen recognized by such CTL clones was previously shown to be encoded by the tyrosinase gene. We report here the identification of another gene that also directs the expression of an antigen recognized on most melanomas by CTL clones that are restricted by HLA-A2. The gene, designated Melan-A, is unrelated to any known gene. It is 18 kb long and comprises five exons. Like the tyrosinase gene, it is expressed in most melanoma tumor samples and, among normal cells, only in melanocytes.
先前已有报道称，在体外经自体肿瘤细胞刺激后，可从HLA - A2黑色素瘤患者的血液淋巴细胞中分离出抗肿瘤细胞溶解性T淋巴细胞（CTL）克隆，并且其中一些CTL克隆可裂解大多数HLA - A2黑色素瘤。先前已表明，此类CTL克隆识别的第一种抗原由酪氨酸酶基因编码。我们在此报告另一个基因的鉴定结果，该基因也指导一种抗原的表达，这种抗原在大多数黑色素瘤上可被受HLA - A2限制的CTL克隆识别。该基因命名为Melan - A，与任何已知基因均无关联。它长18 kb，由五个外显子组成。与酪氨酸酶基因一样，它在大多数黑色素瘤肿瘤样本中表达，而在正常细胞中，仅在黑素细胞中表达。
