Stanley H A, Honigberg B M, Cunningham I
Z Parasitenkd. 1979 Feb 28;58(2):141-9. doi: 10.1007/BF01951338.
Trypanosomes from 14 first-peak parasitemias initiated in mice by injection of in vitro-produced metacyclics were stabilated. Strains derived from these stabilates were analyzed for their antigenic composition by cross-agglutination with immune sera produced in rabbits against 12 of the stabilates. The antigenic composition of the 14 stabilates was compared also with two first-peak parasitemias from mice inoculated with fly-derived metacyclics, the variant-specific antigen of the strain used to initiate the cultures that ultimately became infective, and the antigenic variant that was used to infect the flies. One variant-specific, presumably basic, antigen was found, either as the predominant (nine parasitemias) or as a minor (seven parasitemias) antigen, in all first peak-parasitemia strain initiated with culture- or fly-derived metacyclics; it was absent, however, from the strains (not first-peak parasitemias) used to start the cultures or to infect the flies. Only one of the first-peak parasitemias appeared to have the basic antigen alone. The remaining parasitemia populations seemed to have from about two to six antigens, some of which were common to culture- and fly-derived infections. There was very little, if any, antigenic relationship between the foregoing populations and the strains employed for initiation of cultures or for infection of flies. It is evident from the results that much antigenic similarity exists between the culture- and tsetse fly-derived first-peak parasitemias.
对通过注射体外培养产生的循环后期锥虫而在小鼠体内引发的14次初次高峰寄生虫血症的锥虫进行了稳定化处理。通过与兔针对其中12种稳定化锥虫产生的免疫血清进行交叉凝集，分析了源自这些稳定化锥虫的菌株的抗原组成。还将这14种稳定化锥虫的抗原组成与来自接种采采蝇衍生循环后期锥虫的小鼠的两次初次高峰寄生虫血症、用于启动最终具有感染性的培养物的菌株的变异特异性抗原以及用于感染采采蝇的抗原变异体进行了比较。在所有由培养物或采采蝇衍生的循环后期锥虫引发的初次高峰寄生虫血症菌株中，发现了一种变异特异性的、可能是基础的抗原，它要么是主要抗原（9次寄生虫血症），要么是次要抗原（7次寄生虫血症）；然而，在用于启动培养物或感染采采蝇的菌株（非初次高峰寄生虫血症）中不存在这种抗原。只有一次初次高峰寄生虫血症似乎仅含有基础抗原。其余的寄生虫血症群体似乎含有大约2至6种抗原，其中一些抗原在培养物和采采蝇衍生的感染中是共有的。上述群体与用于启动培养物或感染采采蝇的菌株之间几乎没有抗原关系。从结果可以明显看出，培养物衍生和采采蝇衍生的初次高峰寄生虫血症之间存在很大的抗原相似性。
