Cordes V C, Reidenbach S, Franke W W
Division of Cell Biology, German Cancer Research Center, Heidelberg, Germany.
Cell Tissue Res. 1996 May;284(2):177-91. doi: 10.1007/s004410050578.
We have used antibodies against different proteins of the nuclear pore complex to identify cytoplasmic annulate lamellae by immunocytochemistry at the light- and electron-microscopic level in various lines of cultured mammalian cells. Although annulate lamellae (AL) are seldom observed in some cell lines, they occur in large numbers in other lineages, also with cell-type-specific size distributions. AL are especially abundant and prominent in African green monkey kidney epithelial cells of line RC37 and bovine mammary gland epithelial, cells of line BMGE. We have studied the distribution of AL in relation to other organelles by using double-label immunofluorescence microscopy and have demonstrated a significant relationship between AL and rough endoplasmic reticulum. We have further shown that AL are disassembled during prophase and reassembled at the end of mitosis, almost concomitantly with the disassembly and reassembly of the nuclear envelope. Cultured mammalian cells that are rich in AL can therefore be used as suitable models for studies of the biogenesis of these lamellae, which can conveniently be detected by immunofluorescence microscopy.
我们使用了针对核孔复合体不同蛋白质的抗体，通过免疫细胞化学方法，在光学显微镜和电子显微镜水平上，对多种培养的哺乳动物细胞系中的胞质环层板进行鉴定。尽管在某些细胞系中很少观察到环层板，但在其他细胞谱系中却大量存在，且具有细胞类型特异性的大小分布。环层板在RC37系非洲绿猴肾上皮细胞和BMGE系牛乳腺上皮细胞中尤其丰富且显著。我们通过双标记免疫荧光显微镜研究了环层板与其他细胞器的分布关系，并证明了环层板与粗面内质网之间存在显著关系。我们还进一步表明，环层板在前期解体，并在有丝分裂末期重新组装，几乎与核膜的解体和重新组装同时发生。因此，富含环层板的培养哺乳动物细胞可作为研究这些板层生物发生的合适模型，通过免疫荧光显微镜可方便地检测到它们。
