Sakamoto K, Lawson L D, Milner J A
Department of Nutrition, Pennsylvania State University, University Park 16802, USA.
Nutr Cancer. 1997;29(2):152-6. doi: 10.1080/01635589709514617.
The present studies compared the antiproliferative effects of diallyl trisulfide (DATS) and diallyl disulfide (DADS) on cultured human neoplastic (A549) and nonneoplastic (MRC-5) lung cells. Addition of 10 microM DATS reduced A549 growth by 47%, whereas 10 microM DADS decreased growth by only 20%. DATS treatment (10 microM) did not alter MRC-5 cell growth. DATS (10 microM) caused a marked and progressive increase in intracellular Ca2+ in A549 cells during the first four hours after treatment. Intracellular Ca2+ in A549 cells exposed to DATS returned to near control levels within one hour after refeeding complete medium without DATS. Exposure to 1 microM DATS for 24 hours significantly induced apoptosis, as indicated by increased DNA fragmentation. The ability of DATS and DADS to suppress neoplastic growth is consistent with increasing evidence that several garlic components have anticarcinogenic and antitumorigenic properties.
本研究比较了二烯丙基三硫化物（DATS）和二烯丙基二硫化物（DADS）对培养的人肿瘤性（A549）和非肿瘤性（MRC-5）肺细胞的抗增殖作用。添加10微摩尔的DATS可使A549细胞的生长减少47%，而10微摩尔的DADS仅使生长减少20%。DATS处理（10微摩尔）未改变MRC-5细胞的生长。在处理后的前四个小时内，DATS（10微摩尔）使A549细胞内的Ca2+显著且逐渐增加。在重新加入不含DATS的完全培养基后一小时内，暴露于DATS的A549细胞内的Ca2+恢复到接近对照水平。如DNA片段化增加所示，暴露于1微摩尔DATS 24小时可显著诱导细胞凋亡。DATS和DADS抑制肿瘤生长的能力与越来越多的证据一致，即几种大蒜成分具有抗癌和抗肿瘤特性。
