Adamus G, Aptsiauri N, Guy J, Heckenlively J, Flannery J, Hargrave P A
R.S. Dow Neurological Sciences Institute, Portland, Oregon 97209, USA.
Clin Immunol Immunopathol. 1996 Feb;78(2):120-9. doi: 10.1006/clin.1996.0021.
Cancer-associated retinopathy (CAR) is an uncommon paraneoplastic disease in which degeneration of the retina occurs as a remote effect of cancer in a distant part of the body. Immunoreactivity of sera from CAR patients and controls have been analyzed. Immunostaining of human retinal proteins showed that a soluble protein of Mr approximately 46 kDa (p46) is labeled by antibodies from several CAR patients with various types of cancer (lung, breast, bladder, prostate, salivary gland, and gastrointestinal tract cancer and chronic lymphocytic leukemia). These sera did not show reactivity with the 23-kDa protein previously associated with CAR. To identify and further characterize p46, the retinal protein was purified to homogeneity by anion-exchange chromatography and preparative gel electrophoresis. Protein sequence analysis of the peptides from p46 revealed a high homology with human enolase, an important glycolytic enzyme. Although enolase has been previously identified as a product of several types of tumors, and enolase activity has been detected in the sera of some cancer patients, the existence of autoantibodies directed to enolase has not been described. This is the first report of the presence of serum antibodies to retinal enolase in the patients with cancer and the CAR syndrome. When antibodies of specific isotypes (IgG, IgM, and IgA) were measured, IgG1 isotype was dominant. The significance of these antibodies for the disease process is under investigation.
癌症相关性视网膜病变（CAR）是一种罕见的副肿瘤性疾病，其中视网膜变性是身体远处癌症的一种远隔效应。已对CAR患者和对照者血清的免疫反应性进行了分析。人视网膜蛋白的免疫染色显示，几种患有各种类型癌症（肺癌、乳腺癌、膀胱癌、前列腺癌、唾液腺癌和胃肠道癌以及慢性淋巴细胞白血病）的CAR患者的抗体标记了一种分子量约为46 kDa的可溶性蛋白（p46）。这些血清与先前与CAR相关的23 kDa蛋白没有反应性。为了鉴定并进一步表征p46，通过阴离子交换色谱和制备性凝胶电泳将视网膜蛋白纯化至同质。对来自p46的肽进行的蛋白质序列分析显示与人烯醇化酶高度同源，人烯醇化酶是一种重要的糖酵解酶。尽管烯醇化酶先前已被鉴定为几种类型肿瘤的产物，并且在一些癌症患者的血清中检测到了烯醇化酶活性，但尚未描述针对烯醇化酶的自身抗体的存在。这是关于癌症患者和CAR综合征患者血清中存在针对视网膜烯醇化酶抗体的首次报告。当测量特定同种型（IgG、IgM和IgA）的抗体时，IgG1同种型占主导。这些抗体对疾病进程的意义正在研究中。
