从澳大利亚人和动物身上分离出的鸟分枝杆菌菌株的基因特征分析。

Genetic characterization of Mycobacterium avium isolates recovered from humans and animals in Australia.

作者信息

Feizabadi M M, Robertson I D, Cousins D V, Dawson D, Chew W, Gilbert G L, Hampson D J

机构信息

School of Veterinary Studies, Murdoch University, Western Australia.

出版信息

Epidemiol Infect. 1996 Feb;116(1):41-9. doi: 10.1017/s0950268800058945.

DOI:10.1017/s0950268800058945

PMID:8626003

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2271235/

Abstract

Genetic relationships amongst 115 mainly Australian isolates of Mycobacterium avium were assessed using multilocus enzyme electrophoresis (MEE). The isolates were divided into 58 electrophoretic types (ETs), with a mean genetic diversity of 0.29. Isolates from humans were closely related to but distinct from those cultured from birds, whilst some porcine isolates belonged to the same ETs as certain human isolates. Pulsed field gel electrophoresis (PFGE) was used to differentiate related isolates, and those from birds and some from other animals, including pigs, were distinguished from the human isolates. The results of MEE and PFGE suggested that certain strains of M. avium may be transmitted between birds and pigs, but there was no clear evidence of transmission to humans. The serovar of the M. avium isolates was not obviously related to their ET assignment or their PFGE type.

摘要

利用多位点酶电泳（MEE）评估了115株主要来自澳大利亚的鸟分枝杆菌分离株之间的遗传关系。这些分离株被分为58种电泳类型（ETs），平均遗传多样性为0.29。来自人类的分离株与从鸟类培养的分离株密切相关但又有所不同，而一些猪源分离株与某些人类分离株属于相同的ETs。脉冲场凝胶电泳（PFGE）用于区分相关分离株，来自鸟类和其他一些动物（包括猪）的分离株与人类分离株不同。MEE和PFGE的结果表明，某些鸟分枝杆菌菌株可能在鸟类和猪之间传播，但没有明确证据表明会传播给人类。鸟分枝杆菌分离株的血清型与其ET分类或PFGE类型没有明显关联。