Tanaka M, Tamura K, Ide H
Biological Institute, Tohoku University, Sendai, Japan.
Dev Biol. 1996 May 1;175(2):239-47. doi: 10.1006/dbio.1996.0111.
Exogenously applied retinoic acid (RA) is known to affect cartilage pattern in developing and regenerating limbs. There are, however, few reports which analyze the participation of endogenous RA in limb pattern formation. Using an organ culture system, we attempted to reduce the concentration of endogenous RA in the developing chick wing buds by the treatment with citral (3,7-dimethyl-2,6-octadienal), an inhibitor of retinoic acid formation. After this treatment, the cultured wing buds were grafted to the stumps of host embryos. These citral-treated limb buds frequently formed truncated cartilage elements and the defects were rescued by simultaneous treatment with an appropriate concentration of RA. These results suggest that endogenous RA plays a role in chick limb bud development.
已知外源性应用视黄酸（RA）会影响发育中和再生肢体中的软骨模式。然而，很少有报告分析内源性RA在肢体模式形成中的参与情况。我们使用器官培养系统，尝试通过用视黄酸形成抑制剂柠檬醛（3,7-二甲基-2,6-辛二烯醛）处理来降低发育中的鸡翼芽内源性RA的浓度。经过这种处理后，将培养的翼芽移植到宿主胚胎的残端。这些经柠檬醛处理的肢体芽经常形成截断的软骨元件，并且通过用适当浓度的RA同时处理可以挽救这些缺陷。这些结果表明内源性RA在鸡肢体芽发育中起作用。
