Yeğen E, Akçay F, Yiğitoğlu M R, Celik I, Suzek H, Odabaşioğlu F
Department of Biochemistry, Medical School, Atatürk University, Erzurum, Turkey.
Jpn Heart J. 1995 Nov;36(6):789-95. doi: 10.1536/ihj.36.789.
The effect of alloxan monohydrate-induced diabetes on the resting plasma atrial natriuretic peptide (ANP) level was investigated in 22 male New Zealand white rabbits. Alloxan monohydrate (100 mg/kg) dissolved in saline at a concentration of 50 mg/ml was administered by a single intravenous injection 3 months before the experimental analysis. The diabetic state was examined 72 h later by quantitative determination of blood glucose levels of >350 mg/dl. Beginning on day 3, 14 animals (Group 1) received a daily subcutaneous injection of 1 U insulin having moderate hyperglycemia (blood glucose concentration [BGC] between 300 and 400 mg/dl). Eight animals (Group 2; normoglycemic controls) received 3.2 U of insulin daily to maintain the BGC below 100 mg/dl. Eight healthy rabbits were included in the study as controls (Group 3). Blood samples for ANP analysis were obtained three months after administration of alloxan monohydrate. The plasma ANP levels in moderately diabetic rabbits (328 +/- 43 pg/ml) were significantly higher than those in normoglycemic (98.5 +/- 20 pg/ml) and healthy (76.6 +/- 18 pg/ml) controls (p < 0.001 for both). In addition, we found a significant correlation between plasma levels of glucose and levels of ANP (r = 0.665, p < 0.001). Our data indicate that further experiments need to be performed to investigate what is responsible for the elevation of plasma ANP levels in diabetic rabbits.
在22只雄性新西兰白兔中研究了一水合四氧嘧啶诱导的糖尿病对静息血浆心房利钠肽（ANP）水平的影响。在实验分析前3个月，通过单次静脉注射给予浓度为50mg/ml、溶解于生理盐水中的一水合四氧嘧啶（100mg/kg）。72小时后通过定量测定血糖水平>350mg/dl来检查糖尿病状态。从第3天开始，14只动物（第1组）每天皮下注射1U胰岛素以维持中度高血糖（血糖浓度[BGC]在300至400mg/dl之间）。8只动物（第2组；正常血糖对照组）每天接受3.2U胰岛素以将BGC维持在100mg/dl以下。8只健康兔子作为对照组纳入研究（第3组）。在给予一水合四氧嘧啶3个月后采集用于ANP分析的血样。中度糖尿病兔子的血浆ANP水平（328±43pg/ml）显著高于正常血糖（98.5±20pg/ml）和健康（76.6±18pg/ml）对照组（两者p<0.001）。此外，我们发现血浆葡萄糖水平与ANP水平之间存在显著相关性（r=0.665，p<0.001）。我们的数据表明，需要进行进一步实验来研究导致糖尿病兔子血浆ANP水平升高的原因。
