Kurono Y, Shigemi H, Kodama S, Mogi G
Department of Otolaryngology, Oita Medical University, Japan.
Laryngoscope. 1996 May;106(5 Pt 1):614-8. doi: 10.1097/00005537-199605000-00018.
BALB/c mice were immunized orally or subcutaneously with formalin-killed nontypeable Haemophilus influenzae (NTHi). Salivary immunoglobulin A (IgA) antibody titers against NTHi were significantly increased by oral immunization, but not by subcutaneous immunization. Both immunization procedures remarkably increased the levels of serum antibody activities of both IgA and immunoglobulin G. Live NTHi were then inoculated into the naso-pharynx, and the clearance of the pathogen from the nasopharynx was observed. Significantly fewer bacteria were present in the nasopharynx of the orally immunized mice than in the control mice. However, there was no significant difference between the subcutaneously immunized mice and the control mice. The results indicate that oral immunization can enhance the ability of mice to clear NTHi from the nasopharynx.
用福尔马林灭活的不可分型流感嗜血杆菌（NTHi）经口服或皮下免疫BALB/c小鼠。口服免疫可显著提高唾液中针对NTHi的免疫球蛋白A（IgA）抗体滴度，而皮下免疫则不能。两种免疫程序均显著提高了IgA和免疫球蛋白G的血清抗体活性水平。然后将活的NTHi接种到鼻咽部，并观察病原体从鼻咽部的清除情况。口服免疫小鼠鼻咽部的细菌数量明显少于对照小鼠。然而，皮下免疫小鼠与对照小鼠之间没有显著差异。结果表明，口服免疫可增强小鼠从鼻咽部清除NTHi的能力。
