Miteva K, Valkov N, Goncharova-Peinova J, Kovachev K, Zlatarev S, Pironcheva G, Russev G
Institute of Biology and Immunology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Microbios. 1995;84(339):91-6.
Transcription of ram sperm chromatin was examined by two electron microscopic techniques, namely spreading and thin sections. Labelling by Streptavidin-gold particles permits identification of transcription complexes, that have previously incorporated biotinylated uridine triphosphate. This supports previous electron microscopic data for randomly distributed transcription complexes and the presence of polymerase II molecules, documented by means of specific antibody using immunoelectron microscopy. Labelling of transcripts with biotin permits exact visualization of the transcription process, as well as identification of regions, where transcription occurs.
通过两种电子显微镜技术，即铺展法和超薄切片法，对公羊精子染色质的转录进行了研究。链霉亲和素-金颗粒标记可用于识别先前已掺入生物素化三磷酸尿苷的转录复合物。这支持了先前关于随机分布的转录复合物以及通过免疫电子显微镜使用特异性抗体记录的聚合酶II分子存在的电子显微镜数据。用生物素标记转录本可以准确观察转录过程，并识别发生转录的区域。
