精子中的转录是静止的还是动态的？
Is transcription in sperm stationary or dynamic?
作者信息
Ren Xiaoxia, Chen Xiaoli, Wang Zhenling, Wang Dong
机构信息
The Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing 100193, China.
Beijing Agricultural Vocation College, Beijing 102442, China.
出版信息
J Reprod Dev. 2017 Oct 18;63(5):439-443. doi: 10.1262/jrd.2016-093. Epub 2017 Aug 28.
Abstract
Transcriptional activity is repressed due to the packaging of sperm chromatins during spermiogenesis. The detection of numerous transcripts in sperm, however, raises the question whether transcriptional events exist in sperm, which has been the central focus of the recent studies. To summarize the transcriptional activity during spermiogenesis and in sperm, we reviewed the documents on transcript differences during spermiogenesis, in sperm with differential motility, before and after capacitation and cryopreservation. This will lay a theoretical foundation for studying the mechanism(s) of gene expression in sperm, and would be invaluable in making better use of animal sires and developing reproductive control technologies.
摘要
在精子发生过程中，由于精子染色质的包装，转录活性受到抑制。然而，在精子中检测到大量转录本，这就提出了一个问题，即精子中是否存在转录事件，这一直是近期研究的核心焦点。为了总结精子发生过程中和精子中的转录活性，我们回顾了有关精子发生过程中、不同运动能力的精子中、获能前后以及冷冻保存前后转录本差异的文献。这将为研究精子中基因表达的机制奠定理论基础，并且在更好地利用种畜和开发生殖控制技术方面具有重要价值。
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本文引用的文献
1
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Theriogenology. 2015 Mar 1;83(4):634-41. doi: 10.1016/j.theriogenology.2014.10.027. Epub 2014 Nov 4.
2
Comparative transcript profiling of gene expression of fresh and frozen-thawed bull sperm.新鲜和冻融公牛精子基因表达的比较转录谱分析。
Theriogenology. 2015 Mar 1;83(4):504-11. doi: 10.1016/j.theriogenology.2014.10.015. Epub 2014 Oct 16.
3
Identification and characterization of genes differentially expressed in X and Y sperm using suppression subtractive hybridization and cDNA microarray.利用抑制性消减杂交和cDNA微阵列技术鉴定和表征X精子和Y精子中差异表达的基因。
Mol Reprod Dev. 2014 Oct;81(10):908-17. doi: 10.1002/mrd.22386. Epub 2014 Sep 15.
4
Why do sperm carry RNA? Relatedness, conflict, and control.为什么精子携带 RNA？亲缘关系、冲突与控制。
Trends Ecol Evol. 2014 Aug;29(8):451-5. doi: 10.1016/j.tree.2014.05.006. Epub 2014 Jun 7.
5
The stage-specific testicular germ cell apoptotic response to low-dose X-irradiation and 2,5-hexanedione combined exposure. I: Validation of the laser capture microdissection method for qRT-PCR array application.低剂量X射线照射与2,5-己二酮联合暴露下睾丸生殖细胞的阶段特异性凋亡反应。I：用于qRT-PCR阵列应用的激光捕获显微切割方法的验证。
Toxicol Pathol. 2014 Dec;42(8):1221-8. doi: 10.1177/0192623314526319. Epub 2014 Apr 9.
6
Long noncoding RNAs in spermatogenesis: insights from recent high-throughput transcriptome studies.长非编码 RNA 在精子发生中的作用：来自最近高通量转录组研究的新见解。
Reproduction. 2014 Apr 8;147(5):R131-41. doi: 10.1530/REP-13-0594. Print 2014 May.
7
Genome-scale acetylation-dependent histone eviction during spermatogenesis.精子发生过程中全基因组范围的乙酰化依赖性组蛋白去除
J Mol Biol. 2014 Oct 9;426(20):3342-9. doi: 10.1016/j.jmb.2014.02.023. Epub 2014 Mar 5.
8
T-type Ca2+ channels in spermatogenic cells and sperm.生精细胞和精子中的T型钙离子通道
Pflugers Arch. 2014 Apr;466(4):819-31. doi: 10.1007/s00424-014-1478-2. Epub 2014 Mar 6.
9
Chromatin dynamics during spermiogenesis.精子发生过程中的染色质动力学。
Biochim Biophys Acta. 2014 Mar;1839(3):155-68. doi: 10.1016/j.bbagrm.2013.08.004. Epub 2013 Sep 30.
10
Effect of cryopreservation on human sperm messenger RNAs crucial for fertilization and early embryo development.冷冻保存对人类精子信使 RNA 的影响，这些 RNA 对受精和早期胚胎发育至关重要。
Cryobiology. 2013 Aug;67(1):84-90. doi: 10.1016/j.cryobiol.2013.05.007. Epub 2013 May 31.
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