Sanda S L, Amasino R M
Department of Biochemistry, University of Wisconsin-Madison 53706-1569, USA.
Mol Gen Genet. 1996 Apr 24;251(1):69-74. doi: 10.1007/BF02174346.
The phenotype caused by mutations that affect the timing of flowering in Arabidopsis thaliana has been most extensively analyzed in the Landsberg erecta (Ler) genetic background. In Ler, the late-flowering phenotype of FRIGIDA and mutations in LUMINIDEPENDENS is suppressed by the Ler allele of FLC. In this study, the interactions of nine mutations conferring late flowering with the FLC allele of the Columbia ecotype (FLC-Col), which does not suppress late flowering, were examined. The effect on flowering time of combining six of the mutations with FLC-Col was additive; plants containing FLC-Col with fd, gi, fwa, fha, ft, and fe flowered slightly later than plants containing these mutations with the Ler allele of FLC. In contrast, a synergistic effect was observed between FLC-Col and three mutations; fca, fpa, and fve plants became extremely late flowering when combined with FLC-Col. Maximum delay in flowering for the majority of the mutant strains required FLC-Col in a homozygous state, although for fpa and fe a single copy of FLC-Col allowed maximum lateness. In addition, the fd and fe mutations became more dominant in the presence of FLC-Col.
在拟南芥中，影响开花时间的突变所导致的表型在Landsberg erecta（Ler）遗传背景下得到了最广泛的分析。在Ler中，FRIGIDA的晚花表型以及LUMINIDEPENDENS中的突变会被FLC的Ler等位基因所抑制。在本研究中，检测了九个导致晚花的突变与哥伦比亚生态型的FLC等位基因（FLC-Col）之间的相互作用，该等位基因不会抑制晚花。将其中六个突变与FLC-Col组合对开花时间的影响是累加性的；含有FLC-Col与fd、gi、fwa、fha、ft和fe的植株比含有这些突变与FLC的Ler等位基因的植株开花稍晚。相比之下，在FLC-Col与三个突变之间观察到了协同效应；fca、fpa和fve植株与FLC-Col组合时开花变得极晚。大多数突变株开花的最大延迟需要纯合状态的FLC-Col，尽管对于fpa和fe，一个拷贝的FLC-Col就可导致最大延迟。此外，在存在FLC-Col的情况下，fd和fe突变变得更具显性。
