Clarke J H, Dean C
Department of Molecular Genetics, Cambridge Laboratory, John Innes Centre, Colney, Norwich, UK.
Mol Gen Genet. 1994 Jan;242(1):81-9. doi: 10.1007/BF00277351.
Two loci FRI (FRIGIDA) and KRY (KRYOPHILA) have previously been identified as having major influences on the flowering time of the late-flowering, vernalization-responsive Arabidopsis ecotype, Stockholm. We report here on the mapping and subsequent analysis of these two loci. FRI was mapped to the top of chromosome 4 between markers w122 and m506, using restriction fragment length polymorphism (RFLP) analysis. Due to lack of segregation in of the late-flowering phenotype under the environmental conditions used, KRY could only be localized, by "subtractive genotyping", to chromosome 5 or part of chromosome 3. The map position of FRI indicates that it is not allelic to any of the late-flowering loci identified by mutagenesis of the early-flowering ecotype Landsberg erecta. The late-flowering phenotype conferred by the Stockholm allele of FRI is modified (towards earlier flowering) by Landsberg erecta alleles at an unknown number of loci, perhaps accounting for the absence of fri mutations among mutant lines recovered in Landsberg erecta.
此前已鉴定出两个位点FRI（FRIGIDA）和KRY（KRYOPHILA）对晚花、春化响应型拟南芥生态型斯德哥尔摩的开花时间有重大影响。我们在此报告这两个位点的定位及后续分析。利用限制性片段长度多态性（RFLP）分析，FRI被定位到第4号染色体顶部标记w122和m506之间。由于在所使用的环境条件下晚花表型缺乏分离现象，KRY只能通过“消减基因分型”定位到第5号染色体或第3号染色体的一部分。FRI的图谱位置表明它与通过早花生态型兰茨贝格直立型诱变鉴定出的任何晚花位点都不是等位基因。FRI的斯德哥尔摩等位基因赋予的晚花表型在未知数量的位点上被兰茨贝格直立型等位基因修饰（向早花方向），这可能解释了在兰茨贝格直立型中回收的突变株系中没有fri突变的原因。
