在酶联免疫吸附测定（ELISA）和斑点免疫测定中使用培养滤液抗原诊断类鼻疽病。

Use of culture-filtrated antigen in an ELISA and a dot immunoassay for the diagnosis of melioidosis.

作者信息

Wongratanacheewin S, Amornpunt S, Sermswan R W, Tattawasart U, Wongwajana S

机构信息

Department of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand.

出版信息

Southeast Asian J Trop Med Public Health. 1995 Jun;26(2):329-34.

PMID:8629069

Abstract

An enzyme-linked immunosorbent assay (ELISA) and a dot immunoassay with culture-filtrated antigen were developed for detection of Burkholderia pseudomallei specific antibodies in melioidosis patients. Sixty-eight sera of bacteriologically confirmed melioidosis patients, 45 sera of other bacterial infected patients and 80 sera of healthy blood donors from endemic area were investigated. The samples were subjected to those assays im comparison with indirect hemagglutination (IHA). The sensitivity, specificity, positive and negative predictive values in this dot immunoassay were 94.1%, 99.2%, 98.5% and 96.9%, respectively, with cut-off dilution at 1:4,000, whereas those in ELISA were 92.6%, 96.8%, 94.0% and 96.0%, respectively, with cut-off value of OD = 0.47 at 490 nm. Meanwhile, those in IHA were 64.7%, 93.6%, 84.6%, 83.0% respectively, with a cut-off value of > or = 1:80. The results in this study demonstrated that the dot immunoassay was more reliable and rapid than ELISA as the serological test for diagnosis of melioidosis.

摘要

为检测类鼻疽患者中伯克霍尔德菌属假鼻疽菌特异性抗体，开发了一种酶联免疫吸附测定（ELISA）和一种使用培养滤液抗原的斑点免疫测定法。对68份经细菌学确诊的类鼻疽患者血清、45份其他细菌感染患者血清以及80份来自流行地区的健康献血者血清进行了检测。将这些样本进行上述检测，并与间接血凝试验（IHA）作比较。该斑点免疫测定法的敏感性、特异性、阳性预测值和阴性预测值分别为94.1%、99.2%、98.5%和96.9%，截断稀释度为1:4000，而ELISA的相应值分别为92.6%、96.8%、94.0%和96.0%，490nm处的截断值为OD = 0.47。同时，IHA的相应值分别为64.7%、93.6%、84.6%、83.0%，截断值为≥1:80。本研究结果表明，作为类鼻疽诊断的血清学检测方法，斑点免疫测定法比ELISA更可靠、更快速。