Utans U, Quist W C, McManus B M, Wilson J E, Arceci R J, Wallace A F, Russell M E
Harvard School of Public Health, Department of Pathology, Deaconess Hospital, Boston, Massachussets, USA.
Transplantation. 1996 May 15;61(9):1387-92. doi: 10.1097/00007890-199605150-00018.
Allograft inflammatory factor-1 (AIF-1), a cytokine-responsive macrophage molecule, was originally identified and cloned from rat cardiac allografts with chronic cardiac rejection. We performed the present study to determine whether AIF-1 was also involved in the inflammatory response associated with human cardiac transplant rejection. AIF-1 gene transcripts were identified by the reverse-transcriptase polymerase chain reaction in endomyocardial biopsy specimens from human heart transplants and in macrophage cell lines. In the 441-base pair coding region of the human and rat cDNAs, the nucleotide sequences were 86% identical and the deduced amino acid sequences were 90% identical. Consistent with our studies in the rat. AIF-1 was selectively expressed in human macrophage-like cell lines, and immunostaining in human heart allografts localized the AIF-1 gene product to a subset of CD68+ macrophages in the interstitial and perivascular spaces. The parallels between rat and human AIF-1 expression suggest that AIF-1 may have a common effect on the function of activated macrophages in cardiac allografts.
同种异体移植炎症因子-1（AIF-1）是一种细胞因子反应性巨噬细胞分子，最初是从患有慢性心脏排斥反应的大鼠心脏同种异体移植中鉴定并克隆出来的。我们进行了本研究，以确定AIF-1是否也参与与人类心脏移植排斥反应相关的炎症反应。通过逆转录聚合酶链反应在人类心脏移植的心肌内膜活检标本和巨噬细胞系中鉴定出AIF-1基因转录本。在人类和大鼠cDNA的441个碱基对编码区域中，核苷酸序列的同一性为86%，推导的氨基酸序列同一性为90%。与我们在大鼠中的研究一致，AIF-1在人类巨噬细胞样细胞系中选择性表达，并且在人类心脏同种异体移植中的免疫染色将AIF-1基因产物定位到间质和血管周围空间中的一部分CD68+巨噬细胞。大鼠和人类AIF-1表达之间的相似性表明，AIF-1可能对心脏同种异体移植中活化巨噬细胞的功能具有共同作用。
