番木瓜叶扭曲花叶马铃薯Y病毒衣壳蛋白基因的核苷酸序列

Nucleotide sequence of the capsid protein gene of papaya leaf-distortion mosaic potyvirus.

作者信息

Maoka T, Kashiwazaki S, Tsuda S, Usugi T, Hibino H

机构信息

Okinawa Subtropical Station of the Japan International Research Center for Agricultural Sciences, Okinawa, Japan.

出版信息

Arch Virol. 1996;141(1):197-204. doi: 10.1007/BF01718601.

DOI:10.1007/BF01718601

PMID:8629948

Abstract

The DNA complementary to the 3'-terminal 1 404 nucleotides [excluding the poly(A) tail] of papaya leaf-distortion mosaic potyvirus (PLDMV) RNA was cloned and sequenced. The sequence starts within a long open reading frame (ORF) of 1 195 nucleotides and is followed by a 3' non-coding region of 209 nucleotides. Capsid protein (CP) is encoded at the 3' terminus of the ORF. The CP contains 293 residues and has a Mr of 33 277. The CP of PLDMV exhibits 49 to 59% sequence similarity at the amino acid level to the CPs of papaya ringspot potyvirus (PRSV) and other potyviruses. This result is consistent with the absence of a serological relationship between PLDMV and PRSV or other potyviruses. The results support the assignment of PLDMV as a distinct member of the genus Potyvirus.

摘要

克隆并测序了与番木瓜叶畸形花叶马铃薯Y病毒（PLDMV）RNA 3'末端1404个核苷酸（不包括聚腺苷酸尾）互补的DNA。该序列起始于一个1195个核苷酸的长开放阅读框（ORF）内，随后是一个209个核苷酸的3'非编码区。衣壳蛋白（CP）在ORF的3'末端编码。CP含有293个残基，分子量为33277。PLDMV的CP在氨基酸水平上与番木瓜环斑马铃薯Y病毒（PRSV）和其他马铃薯Y病毒的CP具有49%至59%的序列相似性。这一结果与PLDMV与PRSV或其他马铃薯Y病毒之间不存在血清学关系一致。这些结果支持将PLDMV归为马铃薯Y病毒属的一个独特成员。