Grabowski P S, England A J, Dykhuizen R, Copland M, Benjamin N, Reid D M, Ralston S H
University of Aberdeen, Scotland.
Arthritis Rheum. 1996 Apr;39(4):643-7. doi: 10.1002/art.1780390416.
To develop a simple method for assessing endogenous nitric oxide (NO) production applicable to routine clinical practice in rheumatology.
NO production was assessed in patients with rheumatoid arthritis (RA) as serum nitrate levels and as the urinary nitrate:creatinine ratio in morning samples of urine following an overnight fast. The influence of dietary intake of nitrate on these measurements was investigated in healthy volunteers. The clinical value of the urinary nitrate:creatinine ratio was validated in patients with infectious gastroenteritis, in whom its production is known to be increased.
Urinary nitrate:creatinine ratios were significantly elevated in patients with RA (average 3-fold elevation over controls; P < 0.005) or infectious gastroenteritis (average 10-fold elevation, P < 0.001). Serum nitrate was significantly elevated only in patients with infectious gastroenteritis (P < 0.001). Dietary intake of nitrate had no significant influence on the fasting morning urinary nitrate:creatinine ratio in the healthy volunteers, showing that this parameter is a useful indicator of endogenous NO production.
开发一种适用于风湿病常规临床实践的评估内源性一氧化氮（NO）生成的简单方法。
在类风湿关节炎（RA）患者中，通过血清硝酸盐水平以及禁食过夜后晨尿样本中的尿硝酸盐：肌酐比值来评估NO生成。在健康志愿者中研究硝酸盐饮食摄入对这些测量值的影响。在已知其生成增加的感染性肠胃炎患者中验证尿硝酸盐：肌酐比值的临床价值。
RA患者（平均比对照组升高3倍；P < 0.005）或感染性肠胃炎患者（平均升高10倍，P < 0.001）的尿硝酸盐：肌酐比值显著升高。仅感染性肠胃炎患者的血清硝酸盐显著升高（P < 0.001）。硝酸盐的饮食摄入对健康志愿者禁食晨尿中的尿硝酸盐：肌酐比值无显著影响，表明该参数是内源性NO生成的有用指标。
