Aldebert D, Lamkhioued B, Desaint C, Gounni A S, Goldman M, Capron A, Prin L, Capron M
Centre d'Immunologie et de Biologie Parasitaire, Unité INSERM U167, Institut Pasteur, Lille, France.
Blood. 1996 Mar 15;87(6):2354-60.
Recent reports describe the beneficial use of alpha interferon (IFNalpha) for the treatment of idiopathic hypereosinophilic syndrome (HES) unresponsive to conventional therapy. A clinical improvement associated with a rapid decrease of peripheral blood eosinophilia suggested possible direct effects of IFNalpha on eosinophils through the presence of IFNalpha receptors (IFNalphaR). Reverse transcriptase-polymerase chain reaction (RT-PCR) and cytochemistry were used respectively to detect the presence and define the distribution of IFNalphaR on enriched eosinophil preparations purified from blood cells. IFNalphaR was found on eosinophils collected from patients with various eosinophilic disorders. In addition, IFNalpha inhibited the release of eosinophil granule proteins such as eosinophil cationic protein (ECP), neurotoxin (EDN, or interleukin-5 (IL-5). Moreover, antiparasite cytotoxicity was also strongly reduced in a dose-dependent manner by IFNalpha. These results provide the first evidence that human eosinophils express a functional receptor for IFNalpha and represent a potential basis for the beneficial effects of IFNalpha in patients with hypereosinophilic syndromes.
近期报告描述了α干扰素（IFNα）在治疗对传统疗法无反应的特发性嗜酸性粒细胞增多综合征（HES）中的有益应用。与外周血嗜酸性粒细胞迅速减少相关的临床改善表明，IFNα可能通过存在IFNα受体（IFNαR）而对嗜酸性粒细胞产生直接作用。分别使用逆转录聚合酶链反应（RT-PCR）和细胞化学方法来检测从血细胞中纯化的富集嗜酸性粒细胞制剂上IFNαR的存在并确定其分布。在从各种嗜酸性粒细胞疾病患者收集的嗜酸性粒细胞上发现了IFNαR。此外，IFNα抑制嗜酸性粒细胞颗粒蛋白的释放，如嗜酸性粒细胞阳离子蛋白（ECP）、神经毒素（EDN）或白细胞介素-5（IL-5）。而且，IFNα还以剂量依赖性方式强烈降低抗寄生虫细胞毒性。这些结果首次证明人类嗜酸性粒细胞表达功能性IFNα受体，并为IFNα在嗜酸性粒细胞增多综合征患者中的有益作用提供了潜在依据。
