Piotrowski J J, Shah S, Alexander J J
Case Western Reserve University, Department of Surgery, Cleveland, Ohio 44109, USA.
Life Sci. 1996;58(9):735-40. doi: 10.1016/0024-3205(95)02351-8.
The initial stage of atherosclerotic plaque formation involves oxidation of the phosphatidyl-choline moiety of low density lipoprotein (LDL) and subsequent uptake by macrophages. Ongoing uptake in developing plaque also may involve oxidized LDL and would require an oxidizing environment in plaque lipids. Atherosclerotic plaque lipids from 12 patients undergoing peripheral vascular procedures were extracted in chloroform: methanol (2:1). This extract was applied to a 25 cm 5 micron silica HPLC column and eluted with a ternary gradient mobile phase utilizing a laser light scattering (ELSD) mass detector. Individual lipid fractions were then analyzed. Cholesterol, both free and esterified, was the most prominent lipid in plaque (104 +/- 74 mg/gm tissue. However, lipid peroxides were present in much higher concentrations (3.52 +/- 2.84 FU X 10(4)/mg phospholipid) and overall level (21.27 +/- 10.10 FU X 10(4)/gm plaque) in the phospholipid component (*p< 0.05). Phosphatidyl-choline (PC) accounted for 63% of the total phospholipid peroxides recovered (6.31 +/- 5.09 mg/gm plaque; *p<0.05). PC and phosphatidylinositol (PI) content were linearly related to lipid peroxide fluorescence (PC; r=0.696; p=0.01) (PI; r=0.809; p=0.001). Lipid peroxides in human atherosclerotic plaque are present primarily in the phospholipid component and phosphatidyl-choline forms the bulk of these peroxides. PC may play an important role in ongoing plaque lipid accumulation.
动脉粥样硬化斑块形成的初始阶段涉及低密度脂蛋白（LDL）的磷脂酰胆碱部分氧化，随后被巨噬细胞摄取。在发展中的斑块中持续摄取也可能涉及氧化型LDL，并且需要斑块脂质中的氧化环境。从12名接受外周血管手术的患者中提取动脉粥样硬化斑块脂质，采用氯仿：甲醇（2:1）。将该提取物应用于25 cm 5微米硅胶高效液相色谱柱，并用三元梯度流动相洗脱，使用激光散射（ELSD）质量检测器。然后分析各个脂质组分。游离胆固醇和酯化胆固醇是斑块中最主要的脂质（104±74 mg/g组织）。然而，脂质过氧化物在磷脂组分中的浓度（3.52±2.84 FU×10⁴/mg磷脂）和总体水平（21.27±10.10 FU×10⁴/g斑块）要高得多（*p<0.05）。磷脂酰胆碱（PC）占回收的总磷脂过氧化物的63%（6.31±5.09 mg/g斑块；*p<0.05）。PC和磷脂酰肌醇（PI）含量与脂质过氧化物荧光呈线性相关（PC；r=0.696；p=0.01）（PI；r=0.809；p=0.001）。人类动脉粥样硬化斑块中的脂质过氧化物主要存在于磷脂组分中，磷脂酰胆碱构成了这些过氧化物的大部分。PC可能在斑块脂质的持续积累中起重要作用。
