从成人红细胞膜中分离I抗原并进行部分特性鉴定。
Isolation and partial characterization of I antigen from I adult erythrocyte membranes.
作者信息
Lisowska E, Duk M, Dzierzkowa-Borodej W, Dryjer M
出版信息
Arch Immunol Ther Exp (Warsz). 1978;26(1-6):151-5.
The i-active substance was isolated from i adult and I int erythrocyte membranes by butanol-water extraction and was purified by SDS-gel filtration and thin layer chromatography of peracetylated samples. The i-active substances from both types of erythrocytes showed also IF activity. In antigen isolated from i adult red cells the IF activity was lower than i activity. On the other hand, the antigen of I int red cells showed higher IF than i activity. The chemical characterization of isolated antigens indicated their glycosphingolipid character.
通过丁醇 - 水萃取从i型成人和I型婴儿红细胞膜中分离出i活性物质，并通过过乙酰化样品的SDS - 凝胶过滤和薄层色谱法进行纯化。两种类型红细胞的i活性物质也显示出IF活性。从i型成人红细胞分离出的抗原中，IF活性低于i活性。另一方面，I型婴儿红细胞的抗原显示出比i活性更高的IF活性。分离抗原的化学表征表明它们具有糖鞘脂的性质。
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