Kuusanmäki P, Lauronen J, Paavonen T, Pakarinen M, Savilahti E, Häyry P, Halttunen J
Second Department of Surgery, Helsinki University Central Hospital, Finland.
Scand J Immunol. 1996 May;43(5):566-73. doi: 10.1046/j.1365-3083.1996.d01-71.x.
The change in activity of three disaccharidase enzymes (maltase, sucrase and lactase) was determined according to the method of Dahlqvist during acute rejection in non-immunosuppressed piglet small bowel grafts. In addition, two brush border enzymes, lactase and aminopeptidase, were stained with monoclonal antibodies. Diminishing disaccharidase activity was an early event during rejection. Diminution began 2 days before distinct morphological changes were seen in the mucosal biopsies. Evaluation of disaccharidase activity can thus be used as a confirmatory method in detecting rejection. Reduction in immunohistological staining of lactase and aminopeptidase with monoclonal antibodies and changes in mucosal morphology were observed to progress simultaneously.
在未进行免疫抑制的仔猪小肠移植物急性排斥反应期间，根据达尔奎斯特的方法测定了三种双糖酶（麦芽糖酶、蔗糖酶和乳糖酶）的活性变化。此外，用单克隆抗体对两种刷状缘酶——乳糖酶和氨肽酶进行了染色。双糖酶活性降低是排斥反应早期出现的现象。在黏膜活检中观察到明显形态学变化的2天前，双糖酶活性就开始降低。因此，双糖酶活性评估可用作检测排斥反应的一种辅助方法。观察到乳糖酶和氨肽酶的单克隆抗体免疫组织化学染色减少与黏膜形态变化同时进展。
