Thea D M, Porat R, Nagimbi K, Baangi M, St Louis M E, Kaplan G, Dinarello C A, Keusch G T
New England Medical Center, Boston, Massachusetts, USA.
Ann Intern Med. 1996 Apr 15;124(8):757-62. doi: 10.7326/0003-4819-124-8-199604150-00009.
To examine the relation of circulating cytokines and cytokine antagonists to the progression of human immunodeficiency virus type 1 (HIV-1) disease.
Cross-sectional analysis.
An ambulatory acquired immunodeficiency syndrome (AIDS) research clinic in Kinshasa, Zaire.
48 women with AIDS, 51 women with HIV infection who were clinically asymptomatic, and 11 female controls who did not have HIV infection, all from Zaire.
Plasma levels of interleukin-1beta, tumor necrosis factor-alpha (TNF-alpha), interleukin-6, interleukin-8, interferon-gamma, interleukin-1beta receptor antagonist (interleukin-1Ra), and TNF soluble receptor p55 (TNFsRp55) were assayed by specific radioimmunoassays. Plasma levels of interferon-gamma were assayed by commercial enzyme-linked immunosorbent assay. The Wilcoxon rank-sum test was used to assess the significance of mean and median differences between groups.
Of the 48 patients with AIDS, circulating interleukin-1beta was detected in 2, TNF-alpha in 4, interleukin-6 in 3, and interleukin-8 in 12. None of these factors were seen in any of the 11 controls. Median values of interleukin-1beta (320 pg/mL), TNF-alpha (210 pg/mL), and interleukin-8 (750 pg/mL) were elevated in HIV-infected asymptomatic patients compared with patients with AIDS (2-, 2.6-, and 18.7-fold higher, respectively; P < 0.001). Interleukin-1Ra and TNFsRp55 levels were substantially higher than interleukin-1beta and TNF-alpha levels in HIV-infected asymptomatic patients (73- and 14-fold, respectively) and were higher than those in patients with AIDS (17.8- and 1.74-fold, respectively).
High circulating levels of the proinflammatory cytokines interleukin-1beta and TNF-alpha, combined with an excess of their natural inhibitors interleukin-1Ra and TNF-sRp55, were seen in clinically asymptomatic HIV-1-positive African women but not in African women with AIDS or in HIV-negative controls. Circulating cytokine antagonists may play a clinical role in modulating cytokine-associated symptoms in the early phases of HIV infection.
研究循环细胞因子及细胞因子拮抗剂与1型人类免疫缺陷病毒（HIV-1）疾病进展的关系。
横断面分析。
扎伊尔金沙萨的一家门诊获得性免疫缺陷综合征（AIDS）研究诊所。
48名患AIDS的女性、51名临床无症状的HIV感染女性以及11名未感染HIV的女性对照，均来自扎伊尔。
采用特异性放射免疫分析法检测血浆白细胞介素-1β、肿瘤坏死因子-α（TNF-α）、白细胞介素-6、白细胞介素-8、干扰素-γ、白细胞介素-1β受体拮抗剂（白细胞介素-1Ra）和TNF可溶性受体p55（TNFsRp55）的水平。采用商业酶联免疫吸附测定法检测血浆干扰素-γ水平。采用Wilcoxon秩和检验评估组间均值和中位数差异的显著性。
48例AIDS患者中，2例检测到循环白细胞介素-1β，4例检测到TNF-α，3例检测到白细胞介素-6，12例检测到白细胞介素-8。11名对照中均未检测到这些因子。与AIDS患者相比，HIV感染无症状患者白细胞介素-1β（320 pg/mL）、TNF-α（210 pg/mL）和白细胞介素-8（750 pg/mL）的中位数升高（分别高出2倍、2.6倍和18.7倍；P<0.001）。在HIV感染无症状患者中，白细胞介素-1Ra和TNFsRp55水平显著高于白细胞介素-1β和TNF-α水平（分别高出73倍和14倍），且高于AIDS患者（分别高出17.8倍和1.74倍）。
在临床无症状的HIV-1阳性非洲女性中，可检测到促炎细胞因子白细胞介素-1β和TNF-α的循环水平升高，同时其天然抑制剂白细胞介素-1Ra和TNF-sRp55也过量，但AIDS非洲女性或HIV阴性对照中未出现这种情况。循环细胞因子拮抗剂可能在HIV感染早期调节细胞因子相关症状中发挥临床作用。
