Chollet-Martin S, Simon F, Matheron S, Joseph C A, Elbim C, Gougerot-Pocidalo M A
INSERM U294, Department of Immunology and Biological Haematology, CHU Bichat-Claude Bernard, Paris, France.
AIDS. 1994 Jul;8(7):879-84. doi: 10.1097/00002030-199407000-00003.
To determine plasma cytokine levels [tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta and IL-6] in African patients infected with HIV-2 relative to values in HIV-1-infected patients, and their relation to immunologic and clinical status.
Questions about the observed differences in the pathogenesis of HIV-2 and HIV-1 remain unanswered. Cytokines, especially TNF-alpha, are involved in the regulation of HIV-1 replication, and can be found in the plasma of HIV-1-infected individuals. Therefore, we evaluated TNF-alpha, IL-1 beta and IL-6 levels in the plasma of African patients with different stages of HIV-2 disease. This was a 3-year prospective follow-up study.
Cytokine plasma levels were assayed in 40 HIV-2- and 51 HIV-1-infected patients from Africa. Nineteen of the 40 HIV-2-infected-patients underwent serial assays every 4 months for 3 years. Data were analysed in relation to the number of CD4+ and CD8+ cells, viral load and clinical status.
Plasma levels of TNF-alpha and IL-1 beta were significantly higher in all the HIV-1- and HIV-2-infected patients than in healthy controls; IL-6 levels were around the detection limit for all patients. TNF-alpha levels were lower in the HIV-2-infected than in the HIV-1-infected patients at all Centers for Disease Control and Prevention (CDC) disease stages, including the asymptomatic phase. The CD4+ cell count was always higher in the HIV-2-infected patients, regardless of CDC stage. The prospective follow-up showed that TNF-alpha levels remained stable during the course of HIV-2 disease, as did the CD4+ cell count and virus load.
Lower and stable plasma TNF-alpha levels in African patients infected with HIV-2, associated with lower viral load and higher CD4+ cell count, suggests the existence of a more appropriate and efficient immune response to HIV-2 than to HIV-1.
测定感染HIV-2的非洲患者血浆细胞因子水平[肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β和IL-6]，并与感染HIV-1的患者进行比较，同时研究这些细胞因子水平与免疫和临床状态的关系。
关于HIV-2和HIV-1发病机制中观察到的差异的问题仍未得到解答。细胞因子，尤其是TNF-α，参与HIV-1复制的调节，且可在感染HIV-1的个体血浆中检测到。因此，我们评估了处于不同HIV-2疾病阶段的非洲患者血浆中TNF-α、IL-1β和IL-6的水平。这是一项为期3年的前瞻性随访研究。
对来自非洲的40例感染HIV-2和51例感染HIV-1的患者进行血浆细胞因子水平检测。40例感染HIV-2的患者中有19例在3年中每4个月进行一次系列检测。分析数据与CD4+和CD8+细胞数量、病毒载量及临床状态的关系。
所有感染HIV-1和HIV-2的患者血浆中TNF-α和IL-1β水平均显著高于健康对照；所有患者的IL-6水平均在检测限附近。在包括无症状期在内的所有疾病控制和预防中心（CDC）疾病阶段，感染HIV-2的患者TNF-α水平均低于感染HIV-1的患者。无论CDC阶段如何，感染HIV-2的患者CD4+细胞计数始终较高。前瞻性随访显示，在HIV-2疾病过程中，TNF-α水平、CD4+细胞计数和病毒载量均保持稳定。
感染HIV-2的非洲患者血浆中TNF-α水平较低且稳定，同时病毒载量较低、CD4+细胞计数较高，这表明针对HIV-2存在比HIV-1更合适、更有效的免疫反应。
