变异菌素，一种由变异微球菌产生的含羊毛硫氨酸的新型细菌素：与乳酸乳球菌的乳酸链球菌素481的比较。
Variacin, a new lanthionine-containing bacteriocin produced by Micrococcus varians: comparison to lacticin 481 of Lactococcus lactis.
作者信息
Pridmore D, Rekhif N, Pittet A C, Suri B, Mollet B
机构信息
Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland.
出版信息
Appl Environ Microbiol. 1996 May;62(5):1799-802. doi: 10.1128/aem.62.5.1799-1802.1996.
Abstract
A new lanthionine-containing bacteriocin, variacin, displaying a broad host range of inhibition against gram-positive food spoilage bacteria, has been identified from two strains of Micrococcus varians isolated from meat fermentations. The new bacteriocin was purified, and its amino-terminal end and total amino acid composition were determined. The structural gene was isolated and analyzed. Variacin is resistant to heat and pH conditions from 2 to 10. Its primary sequence shows significant homology to lacticin 481 to Lactococcus lactis, which is more pronounced for the probacteriocin than for the leader sequence. Variacin, like lacticin 481, contains lanthionine and beta-methyllanthionine residues, but its leader sequence clearly resembles nonlantibiotic leader sequences. In particular, the prepeptide contains glycine residues at positions -1 and -2 of the processing site.
摘要
从肉类发酵中分离出的两株变异微球菌中，鉴定出一种新的含羊毛硫氨酸的细菌素——变异菌素，它对革兰氏阳性食品腐败细菌具有广泛的抑制宿主范围。对这种新的细菌素进行了纯化，并测定了其氨基末端和总氨基酸组成。分离并分析了其结构基因。变异菌素对2至10的热和pH条件具有抗性。其一级序列与乳酸乳球菌的Lacticin 481具有显著同源性，对于原细菌素而言这种同源性比对前导序列更为明显。与Lacticin 481一样，变异菌素含有羊毛硫氨酸和β-甲基羊毛硫氨酸残基，但其前导序列明显类似于非羊毛硫抗生素前导序列。特别是，前肽在加工位点的-1和-2位含有甘氨酸残基。
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